Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024223_xyp7 ATATTAACAAACAATAACCAAAATATTGAAAG(+ACTCAC)GTACCATTCACAGAACCATTCCTCAAGTGTA NGG 10 8 17 8 24 0 100 5 10
    240605024222_2cis CTTACCATCACAGTAAGACATAAGTGGGTGAG(+ACTCAC)GATCCCTTCTGTCATTGATTCTTTCTGTAAA NGG 10 8 17 8 24 0 100 5 10
    240605024220_oa7v CACAGTTTACAGAAAGAATCAATGACAGAAGG(+ACTCAC)GATCCTCACCCACTTATGTCTTACTGTGATG NGG 10 8 17 8 24 0 100 5 10
    240605024219_gs4w TCAAAGGCTCAATGCTGCCCTAAATTAAACAG(+ACTCAC)GTACCATGGGCAGTCTAAGTGGGTATTGTGC NGG 10 8 17 8 24 0 100 5 10
    240605024216_sgdb CTCAGGCACAATACCCACTTAGACTGCCCATG(+ACTCAC)GTACCTGTTTAATTTAGGGCAGCATTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605024214_ldr1 TGTGGGAGCAGGTTTCCTGTAGAGCGTTCCAG(+ACTCAC)GTACCACAAGATACAGGGCTCCTATTCAACC NGG 10 8 17 8 24 0 100 5 10
    240605024213_jx6l TTGGGGGTTGAATAGGAGCCCTGTATCTTGTG(+ACTCAC)GTACCTGGAACGCTCTACAGGAAACCTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605024212_3bib AGTTTGACTATAAGAATTTCACTTCTCAACAG(+ACTCAC)GTACCTGAACAGTACAATATTAACGATATTG NGG 10 8 17 8 24 0 100 5 10
    240605024211_faa7 AAGTACAATATCGTTAATATTGTACTGTTCAG(+ACTCAC)GTACCTGTTGAGAAGTGAAATTCTTATAGTC NGG 10 8 17 8 24 0 100 5 10
    240605024209_zuj4 TTGTGGAAGGCAAGGATGTTGGAGAATAGGAG(+ACTCAC)GTACCCAAGCCTCCTGCCTGTCTTAAGGTAG NGG 10 8 17 8 24 0 100 5 10