Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024324_idha CTGAGCGCCTCACTGGGAGATTCTAGGCAGGG(+ACTCAC)GATCCACCACAACCATTCCCTAACTCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240605024323_jfhm GGGGATGCTGCGGACAAGGCATCCAGGATGTG(+ACTCAC)GATCCCCAAGGAGCCTTCAGAAAAGATGCCC NGG 10 8 17 8 24 0 100 5 10
    240605024321_ulzi GGAGAGGGCATCTTTTCTGAAGGCTCCTTGGG(+ACTCAC)GATCCACATCCTGGATGCCTTGTCCGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605024319_5axc TGCAATTATGGGGATCTGAGTTTGAATCCCTG(+ACTCAC)GTACCCATATAAAGACACAGATGCGGTGATG NGG 10 8 17 8 24 0 100 5 10
    240605024317_mu66 GTGCCCATCACCGCATCTGTGTCTTTATATGG(+ACTCAC)GTACCAGGGATTCAAACTCAGATCCCCATAA NGG 10 8 17 8 24 0 100 5 10
    240605024315_b9hz ATCCCCGATCCTAGCCCTGTGTCCTGACCATG(+ACTCAC)GTACCACTGCACCCCACACTTGAGCCACAGG NGG 10 8 17 8 24 0 100 5 10
    240605024314_5sa9 GGGATCCTGTGGCTCAAGTGTGGGGTGCAGTG(+ACTCAC)GTACCATGGTCAGGACACAGGGCTAGGATCG NGG 10 8 17 8 24 0 100 5 10
    240605024311_jpqy CTTCAAGATTGGATTCTGAGCTTAGACCTGGG(+ACTCAC)GTACCCCGCTCCACCTCTCAGTCTTTAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605024309_kpnx AGGTCCCCCTAAAGACTGAGAGGTGGAGCGGG(+ACTCAC)GTACCCCAGGTCTAAGCTCAGAATCCAATCT NGG 10 8 17 8 24 0 100 5 10
    240605024307_asy8 TAATCCTAGCACTCAGGAGGTGAGGCAGGTGG(+ACTCAC)GTACCTGAGTCTGAGGCTAGATTGGTTTAGT NGG 10 8 17 8 24 0 100 5 10