Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024410_h2z5 TCACTGAAACAGAAGAAGCAGTTCCACTTTAG(+ACTCAC)GTACCACAAGGTGTCTTTCTATACAGCTCAT NGG 10 8 17 8 24 0 100 5 10
    240605024409_v5wd CAGACCCCAGTATATTATTTGATCTTATCTTG(+ACTCAC)GATCCTTTTGAAAAGTTAAGAATATATGAAG NGG 10 8 17 8 24 0 100 5 10
    240605024407_tzhm TTTACCTTCATATATTCTTAACTTTTCAAAAG(+ACTCAC)GATCCAAGATAAGATCAAATAATATACTGGG NGG 10 8 17 8 24 0 100 5 10
    240605024406_92ix TACAGACCTGACTTTCATAGTCAGGAGCTTGG(+ACTCAC)GTACCTGCTTCAGCATCATGGACACTGGGAT NGG 10 8 17 8 24 0 100 5 10
    240605024404_y0nm CTGGAATCCCAGTGTCCATGATGCTGAAGCAG(+ACTCAC)GTACCCAAGCTCCTGACTATGAAAGTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240605024403_n8kp AAGCTCATGTTCTGACTGCAAGGATCATGTTG(+ACTCAC)GTACCTACCCTTTAGGAAACTTGCCAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240605024401_ei6i AATTTCTGACTGGCAAGTTTCCTAAAGGGTAG(+ACTCAC)GTACCAACATGATCCTTGCAGTCAGAACATG NGG 10 8 17 8 24 0 100 5 10
    240605024400_owxd CTTGGGTCTAGGGTGAATTCATCTTCTGCTAG(+ACTCAC)GTACCCATGGCCTGCAAGTAGATGCCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605024358_6c5f AGCCCTGCCTGGCATCTACTTGCAGGCCATGG(+ACTCAC)GTACCTAGCAGAAGATGAATTCACCCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240605024356_kfsq ATCTGAAAATGTAATGGCTGCACTCATTCATG(+ACTCAC)GATCCATCTGTCACCCTTCAGACATTTTAGA NGG 10 8 17 8 24 0 100 5 10