Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024355_svto GAGTGTCTAAAATGTCTGAAGGGTGACAGATG(+ACTCAC)GATCCATGAATGAGTGCAGCCATTACATTTT NGG 10 8 17 8 24 0 100 5 10
    240605024354_8zh0 ACATGTAAATTAATATTTAGAGTGAAGGAATG(+ACTCAC)GATCCAATTGATGAAGGAAGATGAGGAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605024353_3geo TGATAGGCTTCCTCATCTTCCTTCATCAATTG(+ACTCAC)GATCCATTCCTTCACTCTAAATATTAATTTA NGG 10 8 17 8 24 0 100 5 10
    240605024352_cyef ACCGTTTCGCTAATAGTTACTGCCAGCAGCTG(+ACTCAC)GATCCTGCATACAAAACACTTGTTCATGTTT NGG 10 8 17 8 24 0 100 5 10
    240605024350_aiy1 AAGGAAAACATGAACAAGTGTTTTGTATGCAG(+ACTCAC)GATCCAGCTGCTGGCAGTAACTATTAGCGAA NGG 10 8 17 8 24 0 100 5 10
    240605024349_fxkz GTCTGCAGTGGGCAGGGTGCTGGAGGAGAAGG(+ACTCAC)GATCCAGGCAGACCCCACTCCAACTGAACCA NGG 10 8 17 8 24 0 100 5 10
    240605024347_2bt7 GTATGTGGTTCAGTTGGAGTGGGGTCTGCCTG(+ACTCAC)GATCCCTTCTCCTCCAGCACCCTGCCCACTG NGG 10 8 17 8 24 0 100 5 10
    240605024345_nz2s GCATTTTTTTTCCATAGGTAACTAACACCATG(+ACTCAC)GATCCAACCAGGCAGAAGTTTGGAGCAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605024343_vs78 AGCAAAACTTGCTCCAAACTTCTGCCTGGTTG(+ACTCAC)GATCCATGGTGTTAGTTACCTATGGAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605024342_rny6 AGACCCTATACAAGCAGCATCGCTGTTGCCAG(+ACTCAC)GATCCTCTTCCTAATGAAGGGTCGCAGAGAC NGG 10 8 17 8 24 0 100 5 10