Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605024252_arky CTGAGGCCCTTCTGTAGCAGGCACAGTGCTAG(+ACTCAC)GATCCCCCTGGTGGACACCACAGGAATTGCA NGG 10 8 17 8 24 0 100 5 10
    240605024251_i6r4 GCTGGCCCCACTCCCTGAGTCTGGCCAAGTTG(+ACTCAC)GATCCTGCTGGTCCTGTTACCAGTTACCTAC NGG 10 8 17 8 24 0 100 5 10
    240605024249_3wb0 CCACTGTAGGTAACTGGTAACAGGACCAGCAG(+ACTCAC)GATCCAACTTGGCCAGACTCAGGGAGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605024248_fjuv TAAATAATTATTTAAAAGACAGGAAGTAAGCG(+ACTCAC)GTACCGAGCAATGAAACTGAAGGTACAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605024247_v9xm GCCCAGTGCTGTACCTTCAGTTTCATTGCTCG(+ACTCAC)GTACCGCTTACTTCCTGTCTTTTAAATAATT NGG 10 8 17 8 24 0 100 5 10
    240605024245_m4tn TGGGCACCTCTGCCCTGCCTGTCTGCTGCCCG(+ACTCAC)GATCCTCCTCACTTAGCACCTAGGGGTGTCA NGG 10 8 17 8 24 0 100 5 10
    240605024243_a6kp GACCCTGACACCCCTAGGTGCTAAGTGAGGAG(+ACTCAC)GATCCGGGCAGCAGACAGGCAGGGCAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240605024242_lswb CTGCTATACCCTGGGAAGGACAACCATCAGTG(+ACTCAC)GTACCTTAGGGGCCTGAGGTTCCAGGGGATC NGG 10 8 17 8 24 0 100 5 10
    240605024240_hs69 CTTTAGATCCCCTGGAACCTCAGGCCCCTAAG(+ACTCAC)GTACCACTGATGGTTGTCCTTCCCAGGGTAT NGG 10 8 17 8 24 0 100 5 10
    240605024239_a0wi CACATGCGTGGTCAGTGTCAGTTACCAACTCG(+ACTCAC)GTACCATTCAAAGAGAAAAGATGGATTTGTT NGG 10 8 17 8 24 0 100 5 10