Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605023451_lniz GACCTGTGAAGACAGGAATGGTCCCCACTCTG(+ACTCAC)GATCCCCTTTGCCACTCCTGGGGAGCCCACC NGG 10 8 17 8 24 0 100 5 10
    240605023449_uz0f AACCACAGGAGGGCAGGGAGGCCTGGTTAGGG(+ACTCAC)GTACCCACACAAAGAAGAGCTCCAACAACTG NGG 10 8 17 8 24 0 100 5 10
    240605023447_krn2 CTGCCCAGTTGTTGGAGCTCTTCTTTGTGTGG(+ACTCAC)GTACCCCTAACCAGGCCTCCCTGCCCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605023446_k0cm ATAATTACCCCAATTCTCCCTAAAACAATAAG(+ACTCAC)GATCCTTGGTAGGTGACTGCAAAATGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605023444_hyep ACTCAGCCTGCATTTTGCAGTCACCTACCAAG(+ACTCAC)GATCCTTATTGTTTTAGGGAGAATTGGGGTA NGG 10 8 17 8 24 0 100 5 10
    240605023443_3bs1 GAGAAGGGCCCACACTTCTTTGTACCTTCTGG(+ACTCAC)GTACCAGTCTGTTCCCAGGTCCCTTACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605023442_y9j7 ACTTGCTGGGTAAGGGACCTGGGAACAGACTG(+ACTCAC)GTACCCAGAAGGTACAAAGAAGTGTGGGCCC NGG 10 8 17 8 24 0 100 5 10
    240605023441_g8ws ACAGCCCCTGTATCCTGATGTTGTCAGGAGTG(+ACTCAC)GTACCCAAGGCCTCCTCTCTGCTCCTTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605023438_0lt3 TGACTCCACAAGGAGCAGAGAGGAGGCCTTGG(+ACTCAC)GTACCACTCCTGACAACATCAGGATACAGGG NGG 10 8 17 8 24 0 100 5 10
    240605023437_7b0y GTCTATGCTGATGCACAGGTTGGTTATGTTTG(+ACTCAC)GTACCACAAGGTGTTTATAATTCAAACAAAA NGG 10 8 17 8 24 0 100 5 10