Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022713_cy2t TCAGCACCCACATGGTAGGGTAGTTCACAAGG(+ACTCAC)GATCCTGCCCTATTCTGACCTGTACCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240605022711_hooz GGCTGATACACTGAGTGCAAGAGTACTGGAGG(+ACTCAC)GATCCAGTGAGTCAAACCAGTAAGCAGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605022710_2dla CACACCTCACTGCTTACTGGTTTGACTCACTG(+ACTCAC)GATCCCTCCAGTACTCTTGCACTCAGTGTAT NGG 10 8 17 8 24 0 100 5 10
    240605022709_fpkx CATTATCTTTGACAATTTAAACAAGATCAAAG(+ACTCAC)GTACCCAAAGGGTTCTGGGAGCTAAGATCTC NGG 10 8 17 8 24 0 100 5 10
    240605022707_ij0m CTATGGAGATCTTAGCTCCCAGAACCCTTTGG(+ACTCAC)GTACCTTTGATCTTGTTTAAATTGTCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605022706_m59e CATTATCTTTGACAATTTAAACAAGATCAAAG(+ACTCAC)GTACCCAAAGGGTTCTGGGAGCTAAGATCTC NGG 10 8 17 8 24 0 100 5 10
    240605022704_rtj8 CTATGGAGATCTTAGCTCCCAGAACCCTTTGG(+ACTCAC)GTACCTTTGATCTTGTTTAAATTGTCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605022703_xkzc GTTTTCTGAAGTCATGGATTAAGTGATCTGCG(+ACTCAC)GATCCACCCAGGCACAGTCAGATCAAAGCGT NGG 10 8 17 8 24 0 100 5 10
    240605022701_5ndp TTGCAACGCTTTGATCTGACTGTGCCTGGGTG(+ACTCAC)GATCCGCAGATCACTTAATCCATGACTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605022658_w4bg GGGAGTCTGAGTTCCTCTCCTGATGTCTAGGG(+ACTCAC)GTACCAGGTTCGCATGTAGTACAAACACATG NGG 10 8 17 8 24 0 100 5 10