Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022609_jszc TTAATGCATAGAACAGAACACTCTCCACTCTG(+ACTCAC)GATCCATGGAATTTTGTGCTTCCACAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240605022607_mfch TGTTAGCGAAACACCTTTAAAGATTTATAAGG(+ACTCAC)GATCCCAGGTAATTAGTATATATGAAATTTT NGG 10 8 17 8 24 0 100 5 10
    240605022605_qhwd CAATAAAAATTTCATATATACTAATTACCTGG(+ACTCAC)GATCCCTTATAAATCTTTAAAGGTGTTTCGC NGG 10 8 17 8 24 0 100 5 10
    240605022603_tg81 GTCTGAGAGCTCTGCACGGGCACAGATGCTGG(+ACTCAC)GTACCTCTTATGGGTGTGGCAGTATTACACG NGG 10 8 17 8 24 0 100 5 10
    240605022602_yco1 TGTTTCGTGTAATACTGCCACACCCATAAGAG(+ACTCAC)GTACCCAGCATCTGTGCCCGTGCAGAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605022600_0bay GTCTGAGAGCTCTGCACGGGCACAGATGCTGG(+ACTCAC)GTACCTCTTATGGGTGTGGCAGTATTACACG NGG 10 8 17 8 24 0 100 5 10
    240605022559_tnzl TGTTTCGTGTAATACTGCCACACCCATAAGAG(+ACTCAC)GTACCCAGCATCTGTGCCCGTGCAGAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605022557_o5wn TTCAAGCAACATATAAAATGAAGAGTTTCCTG(+ACTCAC)GTACCTATCATAGTATTTACATTCCCTTATC NGG 10 8 17 8 24 0 100 5 10
    240605022556_1a6l TTTGAGATAAGGGAATGTAAATACTATGATAG(+ACTCAC)GTACCAGGAAACTCTTCATTTTATATGTTGC NGG 10 8 17 8 24 0 100 5 10
    240605022554_4q7d GGTGATCAACTTGACTTAAAATTGACCCTATG(+ACTCAC)GATCCAAAAGATGATGATGCTAAACAGTATT NGG 10 8 17 8 24 0 100 5 10