Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022507_k0hi ATGTTCTTTGTACCCACGCAGGGTTCTGTCTG(+ACTCAC)GTACCCCACATGTCAGCTTAAAGGACTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605022505_55uu AGTTAAAGAAGTCCTTTAAGCTGACATGTGGG(+ACTCAC)GTACCAGACAGAACCCTGCGTGGGTACAAAG NGG 10 8 17 8 24 0 100 5 10
    240605022504_lrff AGTGTCTGGGTTTGGTGGTTGTTTATGGGATG(+ACTCAC)GATCCCTAGGTAGTGCAGTCTCTGGATGGCC NGG 10 8 17 8 24 0 100 5 10
    240605022502_8uyq GGAATGGCCATCCAGAGACTGCACTACCTAGG(+ACTCAC)GATCCATCCCATAAACAACCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605022500_tslf TACTGTTCCAGGTGTGGAGGGGGAATGGAGAG(+ACTCAC)GTACCTGGTAGGCATCCACAGGGGACATGAT NGG 10 8 17 8 24 0 100 5 10
    240605022459_u7bf CTGAGATCATGTCCCCTGTGGATGCCTACCAG(+ACTCAC)GTACCTCTCCATTCCCCCTCCACACCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240605022457_0lzm CAGTTTAAATTAAGGAGAATATTTAGTGTGGG(+ACTCAC)GATCCTGTTCTGTGGAAAATTTGTCCATTCT NGG 10 8 17 8 24 0 100 5 10
    240605022455_l9kv CACTGAGAATGGACAAATTTTCCACAGAACAG(+ACTCAC)GATCCCCACACTAAATATTCTCCTTAATTTA NGG 10 8 17 8 24 0 100 5 10
    240605022454_skyc ATGTGCCTTTTTCCTTTGCTCACTCTGCTTTG(+ACTCAC)GATCCGTTCACTCTAATAAATCAGTAGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605022453_us5n GTTCTTTCACTACTGATTTATTAGAGTGAACG(+ACTCAC)GATCCAAAGCAGAGTGAGCAAAGGAAAAAGG NGG 10 8 17 8 24 0 100 5 10