Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022403_f8vj GCTAAAGGGTCACTGTGAAAGGACACTGCAAG(+ACTCAC)GATCCCCAGAGGCAGACACCTACAGTGAGTG NGG 10 8 17 8 24 0 100 5 10
    240605022401_s5bq GAAGTCACTCACTGTAGGTGTCTGCCTCTGGG(+ACTCAC)GATCCTTGCAGTGTCCTTTCACAGTGACCCT NGG 10 8 17 8 24 0 100 5 10
    240605022400_r0hp GGATGGGGAGATGTGCAGGGTGAGGGCAATTG(+ACTCAC)GTACCACAATGCCAAGGTCATGAAGCTAGGA NGG 10 8 17 8 24 0 100 5 10
    240605022359_ozoq CTCTGTCCTAGCTTCATGACCTTGGCATTGTG(+ACTCAC)GTACCAATTGCCCTCACCCTGCACATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605022358_usns ACATGGCTGTCTCTCAGCAGCCATGACTGTAG(+ACTCAC)GTACCTAGGTTTCATACAGCTCCATGTAGGC NGG 10 8 17 8 24 0 100 5 10
    240605022356_ui0z CTTATGCCTACATGGAGCTGTATGAAACCTAG(+ACTCAC)GTACCTACAGTCATGGCTGCTGAGAGACAGC NGG 10 8 17 8 24 0 100 5 10
    240605022354_50bq ACTGAGACCTGGACCTGATGCTTGAAAGGCGG(+ACTCAC)GTACCTTATTGACTGAGCTATGTCAACAGTT NGG 10 8 17 8 24 0 100 5 10
    240605022353_f7cb TCTTGAACTGTTGACATAGCTCAGTCAATAAG(+ACTCAC)GTACCCGCCTTTCAAGCATCAGGTCCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605022351_lzv4 GAATTCAGTTAGCAACACTACAGGATATCCTG(+ACTCAC)GTACCAATGAATCTGATAATTCTAAATTAAA NGG 10 8 17 8 24 0 100 5 10
    240605022350_10b5 CAAGTTTTAATTTAGAATTATCAGATTCATTG(+ACTCAC)GTACCAGGATATCCTGTAGTGTTGCTAACTG NGG 10 8 17 8 24 0 100 5 10