Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022334_a5oa GTTAGGCCTACTGTGAAGATTTTTCTTTGTTG(+ACTCAC)GTACCTTTCGGAGTTGTCTCAGAAAGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240605022332_3tot ATCAAAACCCCTTTCTGAGACAACTCCGAAAG(+ACTCAC)GTACCAACAAAGAAAAATCTTCACAGTAGGC NGG 10 8 17 8 24 0 100 5 10
    240605022331_at49 ACAACAGGATAACCAGAAGGAGTTCAGGTGGG(+ACTCAC)GATCCAATTTTGATGATATCACAGAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605022329_dxxl GATCTCTGGCTTCTGTGATATCATCAAAATTG(+ACTCAC)GATCCCCACCTGAACTCCTTCTGGTTATCCT NGG 10 8 17 8 24 0 100 5 10
    240605022328_0a0f AGTTGCCATGTGGGAACTGGGAACTGGAGGTG(+ACTCAC)GATCCTCTAGAAGAGCAGCTAGTGCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605022327_ge4b AGTGGTTAAGAGCACTAGCTGCTCTTCTAGAG(+ACTCAC)GATCCACCTCCAGTTCCCAGTTCCCACATGG NGG 10 8 17 8 24 0 100 5 10
    240605022325_srj5 TCTGTAACAGAATTTTCAGTAGAAAGGCCAAG(+ACTCAC)GATCCTTGATTTGTCAATGGTCACCATAGTG NGG 10 8 17 8 24 0 100 5 10
    240605022324_5knd CTTATCACTATGGTGACCATTGACAAATCAAG(+ACTCAC)GATCCTTGGCCTTTCTACTGAAAATTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240605022323_s8qu TTACAGATTGCTTAAGGACCTGGGCAGCTGTG(+ACTCAC)GATCCACTGGGCTCCTGGGAATAGATGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605022321_djlj GAAGTTCTGCATCTATTCCCAGGAGCCCAGTG(+ACTCAC)GATCCACAGCTGCCCAGGTCCTTAAGCAATC NGG 10 8 17 8 24 0 100 5 10