Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022249_1syd CACTAAAACTGGCTCACCTCATCTAGGTTAGG(+ACTCAC)GTACCTATCGTTGAGTTTATTAGACAGTTAG NGG 10 8 17 8 24 0 100 5 10
    240605022248_bipm CAACTCTAACTGTCTAATAAACTCAACGATAG(+ACTCAC)GTACCCTAACCTAGATGAGGTGAGCCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605022246_i06p TGCTAGAGTTATTGTTACAATAATACAGGCCG(+ACTCAC)GTACCTACTGTACAGAGTTAAAACTATATGG NGG 10 8 17 8 24 0 100 5 10
    240605022244_6pd1 TAAAGCCATATAGTTTTAACTCTGTACAGTAG(+ACTCAC)GTACCGGCCTGTATTATTGTAACAATAACTC NGG 10 8 17 8 24 0 100 5 10
    240605022242_w2zw CCCCTTAACACCATCTCCAGCCTCATCCTAGG(+ACTCAC)GTACCAGCTCAAAACAATACCGGGAGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240605022241_ccc5 CCGCCTGCTTCTCCCGGTATTGTTTTGAGCTG(+ACTCAC)GTACCCTAGGATGAGGCTGGAGATGGTGTTA NGG 10 8 17 8 24 0 100 5 10
    240605022240_mld3 AGCAAGCTCGGGAGCTCCCAGAATCTGCTTAG(+ACTCAC)GATCCACTCCAGGGCTTGGAGAGGCGATGAA NGG 10 8 17 8 24 0 100 5 10
    240605022239_925b GTTTGTTCATCGCCTCTCCAAGCCCTGGAGTG(+ACTCAC)GATCCTAAGCAGATTCTGGGAGCTCCCGAGC NGG 10 8 17 8 24 0 100 5 10
    240605022237_kaq8 AGACCTGGATCTCACTTGGATAAGAGTTCTGG(+ACTCAC)GATCCTGCCATGATCCTTGAGAAAGAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605022236_vwm0 GCACGATTTTTCTTTCTCAAGGATCATGGCAG(+ACTCAC)GATCCCAGAACTCTTATCCAAGTGAGATCCA NGG 10 8 17 8 24 0 100 5 10