Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022233_bwlu ACAGGGCCTTACTGGCTCAGGGATTTGCCTGG(+ACTCAC)GTACCTATGATTTCTCTTTCCCTACCCCATG NGG 10 8 17 8 24 0 100 5 10
    240605022232_m6sz ATCAACATGGGGTAGGGAAAGAGAAATCATAG(+ACTCAC)GTACCCAGGCAAATCCCTGAGCCAGTAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605022230_0eou AGGTTAGCAGTGGCAAGCTCACATAGAAGTGG(+ACTCAC)GTACCACAGGGGTGAAGTAAGAGGTGCACCC NGG 10 8 17 8 24 0 100 5 10
    240605022228_t1hc TCTGTGGGTGCACCTCTTACTTCACCCCTGTG(+ACTCAC)GTACCCACTTCTATGTGAGCTTGCCACTGCT NGG 10 8 17 8 24 0 100 5 10
    240605022226_8pdb CCTTAAGCTGTACTACCCTTTTCTAGACATGG(+ACTCAC)GATCCAGGTGTCCTGGTATCAGAGATCCTTG NGG 10 8 17 8 24 0 100 5 10
    240605022225_5xt7 GAGGGCAAGGATCTCTGATACCAGGACACCTG(+ACTCAC)GATCCCATGTCTAGAAAAGGGTAGTACAGCT NGG 10 8 17 8 24 0 100 5 10
    240605022223_jblg TAATCTCAGTGCTGTGGAGGCAGAGACAGGAG(+ACTCAC)GATCCCTGAGCAGAAAAGCCCTACTGATGAG NGG 10 8 17 8 24 0 100 5 10
    240605022222_z75a TGGAGCTCATCAGTAGGGCTTTTCTGCTCAGG(+ACTCAC)GATCCTCCTGTCTCTGCCTCCACAGCACTGA NGG 10 8 17 8 24 0 100 5 10
    240605022220_4c16 CTCAGGGAAGAGTGGTCACAGAGGGACAGAGG(+ACTCAC)GATCCCACCCCCACCCCCACCCCCACCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605022218_rj43 ATCGCTCCAGGTGGGGGTGGGGGTGGGGGTGG(+ACTCAC)GATCCCTCTGTCCCTCTGTGACCACTCTTCC NGG 10 8 17 8 24 0 100 5 10