Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022133_6z6r ACCACATGGTGGCTCACAACCATCTGTAATGG(+ACTCAC)GATCCGATGCCCTCTTCTGGTGATGCTATTT NGG 10 8 17 8 24 0 100 5 10
    240605022131_hw97 ACAGAAAATAGCATCACCAGAAGAGGGCATCG(+ACTCAC)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605022130_1szf GGATCTAGGGAATGAATAGTAGACCGTCAGTG(+ACTCAC)GTACCATTACGTACAGTTGGTTACAGCACCG NGG 10 8 17 8 24 0 100 5 10
    240605022129_4u13 TGGCACGGTGCTGTAACCAACTGTACGTAATG(+ACTCAC)GTACCACTGACGGTCTACTATTCATTCCCTA NGG 10 8 17 8 24 0 100 5 10
    240605022128_0u0p CCAGGCAACAGAAACGCACATTAATGGTGACG(+ACTCAC)GTACCGCTCACATGTGCTCACTACGAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240605022126_9ayp TGTGAGACACTCGTAGTGAGCACATGTGAGCG(+ACTCAC)GTACCGTCACCATTAATGTGCGTTTCTGTTG NGG 10 8 17 8 24 0 100 5 10
    240605022125_axvs TCATGTATAAATGTAGACCATGCATGTGCCTG(+ACTCAC)GTACCTGCAGAAGCCAGTAGAGTGCAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605022123_y36f AAGGGTCCACTGCACTCTACTGGCTTCTGCAG(+ACTCAC)GTACCAGGCACATGCATGGTCTACATTTATA NGG 10 8 17 8 24 0 100 5 10
    240605022122_1pop GTCCAAGTGTAGGCAACCTTTCTTGGTTGATG(+ACTCAC)GATCCAGTCTCTTGTGACAGCTGTCTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605022121_knnn CAGTGCCCACAGACAGCTGTCACAAGAGACTG(+ACTCAC)GATCCATCAACCAAGAAAGGTTGCCTACACT NGG 10 8 17 8 24 0 100 5 10