Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022217_iy1c GGACAAGGTTTTCAGGTATATCTTTAAACAAG(+ACTCAC)GATCCAAGACTCCGTGTCCTACAGGCTATTG NGG 10 8 17 8 24 0 100 5 10
    240605022215_vu03 TTTATCAATAGCCTGTAGGACACGGAGTCTTG(+ACTCAC)GATCCTTGTTTAAAGATATACCTGAAAACCT NGG 10 8 17 8 24 0 100 5 10
    240605022214_g85u CTGGACCACTTTGGGCTACTGTGTCCCTGAGG(+ACTCAC)GATCCTATCCTAGCTAGGAGCCCTTACAAGT NGG 10 8 17 8 24 0 100 5 10
    240605022213_i44l CTCTGACTTGTAAGGGCTCCTAGCTAGGATAG(+ACTCAC)GATCCCTCAGGGACACAGTAGCCCAAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605022211_4fo5 ATGTGTCCCGGGAAAGCCAGAAGAGAGCTCCG(+ACTCAC)GATCCACTGTAGCTGGAGTCAAAGCAAGATG NGG 10 8 17 8 24 0 100 5 10
    240605022210_zsge GCACCCATCTTGCTTTGACTCCAGCTACAGTG(+ACTCAC)GATCCGGAGCTCTCTTCTGGCTTTCCCGGGA NGG 10 8 17 8 24 0 100 5 10
    240605022208_0b0f CAGGGCAGCCATACCAGGACTTTGGGGCTCAG(+ACTCAC)GTACCTTTTGCCTTTGGAGCCCCTTAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240605022207_7ijj ATTTATGCACTAAGGGGCTCCAAAGGCAAAAG(+ACTCAC)GTACCTGAGCCCCAAAGTCCTGGTATGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605022206_p7z7 ATATTCCCAGCCCTCTTCTTGTTTGAAGGCAG(+ACTCAC)GATCCCACTAAATTGCTCTGGCAGGCCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605022204_cq6p TGAGTTCAAGGCCTGCCAGAGCAATTTAGTGG(+ACTCAC)GATCCTGCCTTCAAACAAGAAGAGGGCTGGG NGG 10 8 17 8 24 0 100 5 10