Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022320_lrux TAGTGAAGACAACTCTGACCTTGGCATAAAAG(+ACTCAC)GATCCAAGTCAGAATCCCAGCTTGGTCAATT NGG 10 8 17 8 24 0 100 5 10
    240605022318_lzey GTATTAATTGACCAAGCTGGGATTCTGACTTG(+ACTCAC)GATCCTTTTATGCCAAGGTCAGAGTTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240605022317_u8jv TAGAGCTGCCCACATCACTGATCCTGCTTATG(+ACTCAC)GATCCTCTCCTCTCTGTGGATGTACCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605022316_asz3 CAGGACACAGGGTACATCCACAGAGAGGAGAG(+ACTCAC)GATCCATAAGCAGGATCAGTGATGTGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605022314_3mrj TAAAAGGAAAAGGGAAGCCACTGCTACTGGAG(+ACTCAC)GATCCCCTTCAGTTTTACAAAAAGCTCATCT NGG 10 8 17 8 24 0 100 5 10
    240605022312_spnx GTTCAAGATGAGCTTTTTGTAAAACTGAAGGG(+ACTCAC)GATCCTCCAGTAGCAGTGGCTTCCCTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605022311_cqra GTGCATCTTACAACAGCTAAGCATTTATTAAG(+ACTCAC)GATCCTCATGAGATACAAGATTAGGGAGACA NGG 10 8 17 8 24 0 100 5 10
    240605022310_gk5x CCACATGTCTCCCTAATCTTGTATCTCATGAG(+ACTCAC)GATCCTTAATAAATGCTTAGCTGTTGTAAGA NGG 10 8 17 8 24 0 100 5 10
    240605022308_at4i ATGGTGGCACTAATTTCTGAAATTCATCCAGG(+ACTCAC)GATCCAAATACTGTTTTTGAGTTCAGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605022306_u8km GCAGGGAAAACTGAACTCAAAAACAGTATTTG(+ACTCAC)GATCCCTGGATGAATTTCAGAAATTAGTGCC NGG 10 8 17 8 24 0 100 5 10