Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022418_y1ax GGTTTGGTAAAGACATGTGACCAGGCATAAAG(+ACTCAC)GATCCTTTCGCTGAGCCTTTAGAAAGGCATT NGG 10 8 17 8 24 0 100 5 10
    240605022416_ld7c CCCTGAATGCCTTTCTAAAGGCTCAGCGAAAG(+ACTCAC)GATCCTTTATGCCTGGTCACATGTCTTTACC NGG 10 8 17 8 24 0 100 5 10
    240605022415_4yov CTTGCCATTAACCAGGGCTCTCCTGAAGACAG(+ACTCAC)GTACCACATTTTCATTCAGGCTTGTCATTGA NGG 10 8 17 8 24 0 100 5 10
    240605022414_qe54 GGAGATCAATGACAAGCCTGAATGAAAATGTG(+ACTCAC)GTACCTGTCTTCAGGAGAGCCCTGGTTAATG NGG 10 8 17 8 24 0 100 5 10
    240605022413_8giz CTGTGACGGAACTTCCCGTTAGCCATATTTTG(+ACTCAC)GTACCCTGCCTTTCGAAAAGGACACTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605022411_xj29 TTGTTTGAGAAGTGTCCTTTTCGAAAGGCAGG(+ACTCAC)GTACCAAAATATGGCTAACGGGAAGTTCCGT NGG 10 8 17 8 24 0 100 5 10
    240605022409_oxs3 AGCATTTGTCTTTTACACTTGTACTGTTTCGG(+ACTCAC)GATCCCAAAGATAATCTGTGAGACCTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240605022408_u197 GGCAAATTTAAGGTCTCACAGATTATCTTTGG(+ACTCAC)GATCCCGAAACAGTACAAGTGTAAAAGACAA NGG 10 8 17 8 24 0 100 5 10
    240605022406_dmny TTCGCAGCGCCCACAGCCGTGTTTTCTGCTTG(+ACTCAC)GATCCCACCCCCTCCCATCCACGGAACGTGG NGG 10 8 17 8 24 0 100 5 10
    240605022405_vdu0 GCAGCCCACGTTCCGTGGATGGGAGGGGGTGG(+ACTCAC)GATCCAAGCAGAAAACACGGCTGTGGGCGCT NGG 10 8 17 8 24 0 100 5 10