Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605022553_1cur AAACTAATACTGTTTAGCATCATCATCTTTTG(+ACTCAC)GATCCATAGGGTCAATTTTAAGTCAAGTTGA NGG 10 8 17 8 24 0 100 5 10
    240605022550_0qru CACCGTAATCATGTTAAGTCCATCACCCAGTG(+ACTCAC)GATCCCTGAGGCTGCCTCGAGGGGATTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605022549_8wbg TTGTCAAAGAATCCCCTCGAGGCAGCCTCAGG(+ACTCAC)GATCCACTGGGTGATGGACTTAACATGATTA NGG 10 8 17 8 24 0 100 5 10
    240605022547_f7ic TGACTTTGAAGTAACTGGCCTTGTGCCCTATG(+ACTCAC)GATCCAAGGGCACAGAATGCAGTTCTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605022544_hgsv TCTTCAAAACAGAACTGCATTCTGTGCCCTTG(+ACTCAC)GATCCATAGGGCACAAGGCCAGTTACTTCAA NGG 10 8 17 8 24 0 100 5 10
    240605022543_sqsb GAGCTGTAAGTGAGTGTACTCCAGGATACAAG(+ACTCAC)GTACCGATATGTCATGGTCCTCTGACGATCA NGG 10 8 17 8 24 0 100 5 10
    240605022542_k7em ACACATGATCGTCAGAGGACCATGACATATCG(+ACTCAC)GTACCTTGTATCCTGGAGTACACTCACTTAC NGG 10 8 17 8 24 0 100 5 10
    240605022541_pvyn TGGATACATCACAGTATGCATGTGAATCGAAG(+ACTCAC)GATCCTTATACATACATAAAGACTTAAATTT NGG 10 8 17 8 24 0 100 5 10
    240605022539_1xch AATACAAATTTAAGTCTTTATGTATGTATAAG(+ACTCAC)GATCCTTCGATTCACATGCATACTGTGATGT NGG 10 8 17 8 24 0 100 5 10
    240605022538_ksnk ACAGCCAGCAAGACTTGGTGGCATTGGGATTG(+ACTCAC)GTACCATTTAACACAGAGAAAGCTCCTTGGA NGG 10 8 17 8 24 0 100 5 10