Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605021957_4p7f TAATGTGTCATGTGTTATATTAATCTCTATTG(+ACTCAC)GTACCTGACATTGTTTCTTTGAGCAGTATGA NGG 10 8 17 8 24 0 100 5 10
    240605021956_fkmg TTGCATCATACTGCTCAAAGAAACAATGTCAG(+ACTCAC)GTACCAATAGAGATTAATATAACACATGACA NGG 10 8 17 8 24 0 100 5 10
    240605021955_buck CTTATGACTTAGAATAATATTTTTTTCACATG(+ACTCAC)GATCCACCAGGGTTAGTTTCAGAGTCAGTAC NGG 10 8 17 8 24 0 100 5 10
    240605021954_l1g8 TTCCAGTACTGACTCTGAAACTAACCCTGGTG(+ACTCAC)GATCCATGTGAAAAAAATATTATTCTAAGTC NGG 10 8 17 8 24 0 100 5 10
    240605021952_pgio AGGCATGCTGTGTCTGAAGTGTTTAATAAAGG(+ACTCAC)GATCCTTGTTACAGAACTTGTCTTCCGATGC NGG 10 8 17 8 24 0 100 5 10
    240605021951_kosm GGTGTGCATCGGAAGACAAGTTCTGTAACAAG(+ACTCAC)GATCCCTTTATTAAACACTTCAGACACAGCA NGG 10 8 17 8 24 0 100 5 10
    240605021948_abxf GTGTTTGTGAAGCCCGAACAGTGTGAGTCCGG(+ACTCAC)GTACCGTGTGTACATACATATGTAGGAATAA NGG 10 8 17 8 24 0 100 5 10
    240605021947_xymx AAACGTTATTCCTACATATGTATGTACACACG(+ACTCAC)GTACCCGGACTCACACTGTTCGGGCTTCACA NGG 10 8 17 8 24 0 100 5 10
    240605021946_yhsj ACCCAATATCTCCTACACCTAGTTGCAATAGG(+ACTCAC)GTACCTGTGCCCTGGACTTTCTCCTTACATG NGG 10 8 17 8 24 0 100 5 10
    240605021944_bz83 TAACCCATGTAAGGAGAAAGTCCAGGGCACAG(+ACTCAC)GTACCCTATTGCAACTAGGTGTAGGAGATAT NGG 10 8 17 8 24 0 100 5 10