Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605021858_m9s7 GCCTGTCATGGTATACCCCTGCTAGCCTTGGG(+ACTCAC)GATCCATAAGGAGACCCCTAGCATGGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605021857_y02u AAGTACAGGGCCATGCTAGGGGTCTCCTTATG(+ACTCAC)GATCCCCAAGGCTAGCAGGGGTATACCATGA NGG 10 8 17 8 24 0 100 5 10
    240605021855_rfy6 TGGCACTGTTACCACTTTTGAAAGGTTGTTGG(+ACTCAC)GTACCAGGGTAATCCCACTAAATGTGGAGTA NGG 10 8 17 8 24 0 100 5 10
    240605021853_79kt AAGTATACTCCACATTTAGTGGGATTACCCTG(+ACTCAC)GTACCCAACAACCTTTCAAAAGTGGTAACAG NGG 10 8 17 8 24 0 100 5 10
    240605021851_29ec TGCTGTGAACTAGAACCGTGGGTAGAGGCCGG(+ACTCAC)GTACCCTGGGGTTTGTGGAGAAGGTGTCACT NGG 10 8 17 8 24 0 100 5 10
    240605021848_crtp GGCACAGTGACACCTTCTCCACAAACCCCAGG(+ACTCAC)GTACCCGGCCTCTACCCACGGTTCTAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240605021846_docx CAGCCCCCATCAAGTCCCTGTGCAAACTGGAG(+ACTCAC)GTACCAGCTGGCTTCAGGATGGGGCACCATA NGG 10 8 17 8 24 0 100 5 10
    240605021845_9v3y GGAGATATGGTGCCCCATCCTGAAGCCAGCTG(+ACTCAC)GTACCTCCAGTTTGCACAGGGACTTGATGGG NGG 10 8 17 8 24 0 100 5 10
    240605021843_nvga CACTTGTGCTGTTGGCTCATATTTTCACCTTG(+ACTCAC)GATCCTCTTACCTGTTGCCCAAGCCTCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605021842_dqwb TGAATCTCAGAGGCTTGGGCAACAGGTAAGAG(+ACTCAC)GATCCAAGGTGAAAATATGAGCCAACAGCAC NGG 10 8 17 8 24 0 100 5 10