Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605021750_l4i7 CTCAGGACCACCTTGCCAAGGAGAATAAACCG(+ACTCAC)GATCCTGTTGCACTCTCCTGTCCATCCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605021748_otga TCTACAAAGGGATGGACAGGAGAGTGCAACAG(+ACTCAC)GATCCGGTTTATTCTCCTTGGCAAGGTGGTC NGG 10 8 17 8 24 0 100 5 10
    240605021747_eb3c CTGCTGGCATTGGGCCTCTGAGGCGGGGTTGG(+ACTCAC)GTACCTTCCTCCTTGGGCCTCATACTACTTA NGG 10 8 17 8 24 0 100 5 10
    240605021745_1n4s AAAACTAAGTAGTATGAGGCCCAAGGAGGAAG(+ACTCAC)GTACCCAACCCCGCCTCAGAGGCCCAATGCC NGG 10 8 17 8 24 0 100 5 10
    240605021743_0l6d TACATGCCTCCCAGTCCCTCCACAGCAACCGG(+ACTCAC)GTACCTGGGGATTTTTCCCAAAGGACTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605021740_u409 TGGCAATGGAGTCCTTTGGGAAAAATCCCCAG(+ACTCAC)GTACCCGGTTGCTGTGGAGGGACTGGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605021739_whsm GGGGCATGCTGGCTTTTCTCTGCCACACTCTG(+ACTCAC)GATCCCTGTGCTCTGCTTGCTGGTGAATGAC NGG 10 8 17 8 24 0 100 5 10
    240605021737_n0si CAGTGGTCATTCACCAGCAAGCAGAGCACAGG(+ACTCAC)GATCCAGAGTGTGGCAGAGAAAAGCCAGCAT NGG 10 8 17 8 24 0 100 5 10
    240605021736_yqyx TTATACAGCCCAGACCTACCTGCCTAGGGATG(+ACTCAC)GTACCACCCGAAGATAGCTACATCAATCAAG NGG 10 8 17 8 24 0 100 5 10
    240605021734_m9ng CTTTTCTTGATTGATGTAGCTATCTTCGGGTG(+ACTCAC)GTACCATCCCTAGGCAGGTAGGTCTGGGCTG NGG 10 8 17 8 24 0 100 5 10