Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605021840_1as4 CACTTGTGCTGTTGGCTCATATTTTCACCTTG(+ACTCAC)GATCCTCTTACCTGTTGCCCAAGCCTCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605021839_87dv TGAATCTCAGAGGCTTGGGCAACAGGTAAGAG(+ACTCAC)GATCCAAGGTGAAAATATGAGCCAACAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605021837_rm4q CCCAATAGTCCTTTCCACTTTAACTCTTCCAG(+ACTCAC)GTACCTTTTGGTCCTGCTGTAGCCACTGGCA NGG 10 8 17 8 24 0 100 5 10
    240605021836_szrl GGGCATGCCAGTGGCTACAGCAGGACCAAAAG(+ACTCAC)GTACCTGGAAGAGTTAAAGTGGAAAGGACTA NGG 10 8 17 8 24 0 100 5 10
    240605021835_4yfr GAGGACAGAGGACAACTCAGAGTATTCTTCAG(+ACTCAC)GTACCCTTCATTTTTCTCACTGGCCCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605021833_bvfe GGCGAGCCCTGGGCCAGTGAGAAAAATGAAGG(+ACTCAC)GTACCTGAAGAATACTCTGAGTTGTCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605021831_te7z CTCCCTCTTACCAACCATATGCATACGCCAAG(+ACTCAC)GATCCTGTCCCTCTAGGTCTGTCAGCTTGCA NGG 10 8 17 8 24 0 100 5 10
    240605021830_eane GAGCATGCAAGCTGACAGACCTAGAGGGACAG(+ACTCAC)GATCCTTGGCGTATGCATATGGTTGGTAAGA NGG 10 8 17 8 24 0 100 5 10
    240605021827_gx3w GCTTGCCCAGTCCCTGCACGGTAGATCCCTTG(+ACTCAC)GTACCTGCAGCTCTCAGGTACGGAAGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605021826_cccc TAGACCTTTCCTTCCGTACCTGAGAGCTGCAG(+ACTCAC)GTACCAAGGGATCTACCGTGCAGGGACTGGG NGG 10 8 17 8 24 0 100 5 10