Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605021808_d147 GCCGGTTTCCGGGCTGGCCCCTCCTACCCCTG(+ACTCAC)GATCCCAGACTGTCCTGGATTCCACCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605021806_ngnv ATGAACCCAGGGTGGAATCCAGGACAGTCTGG(+ACTCAC)GATCCAGGGGTAGGAGGGGCCAGCCCGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605021804_36h7 GGTCCCTGCAGGAGGACCCTCACCTTCACCTG(+ACTCAC)GATCCGCCTTGGCCTCTACCCTTGCCCAACC NGG 10 8 17 8 24 0 100 5 10
    240605021803_z6l3 TGGGAGGTTGGGCAAGGGTAGAGGCCAAGGCG(+ACTCAC)GATCCAGGTGAAGGTGAGGGTCCTCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605021800_700a TAACAGGCCACATTCTGATCTTCCAACCGCTG(+ACTCAC)GATCCCTTCCTCCACCTGGCTTCTCAGCTCC NGG 10 8 17 8 24 0 100 5 10
    240605021759_zi9y CATGGGGAGCTGAGAAGCCAGGTGGAGGAAGG(+ACTCAC)GATCCAGCGGTTGGAAGATCAGAATGTGGCC NGG 10 8 17 8 24 0 100 5 10
    240605021757_t2cb AAGAGGAGATTTTTTACAAAGTTTCAGTGTGG(+ACTCAC)GATCCCTAGGCACTGCCATCACTCAGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240605021755_g9fo TGACTTCTTGCTGAGTGATGGCAGTGCCTAGG(+ACTCAC)GATCCCACACTGAAACTTTGTAAAAAATCTC NGG 10 8 17 8 24 0 100 5 10
    240605021753_s5hu TGGCGCCAACATGACCAGAGTCACTAGGAAAG(+ACTCAC)GATCCTAGGTAGCACAGACACAAACCATGTC NGG 10 8 17 8 24 0 100 5 10
    240605021751_0fe2 CCCAGGACATGGTTTGTGTCTGTGCTACCTAG(+ACTCAC)GATCCTTTCCTAGTGACTCTGGTCATGTTGG NGG 10 8 17 8 24 0 100 5 10