Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605021546_jinx TGGTGGGAGGGTGGGAACTGGGGTATTCTTAG(+ACTCAC)GTACCTCTGACATTGTAGCAGCTCCTTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605021545_yvi9 CACCCTTTGAAGGAGCTGCTACAATGTCAGAG(+ACTCAC)GTACCTAAGAATACCCCAGTTCCCACCCTCC NGG 10 8 17 8 24 0 100 5 10
    240605021544_4akj CAAAAGACTTTCAGGGGGACCTTCCTTGTTGG(+ACTCAC)GATCCAATGGCTGTGGCTGATGGAACCAATC NGG 10 8 17 8 24 0 100 5 10
    240605021542_1ti8 TTAAAGATTGGTTCCATCAGCCACAGCCATTG(+ACTCAC)GATCCCAACAAGGAAGGTCCCCCTGAAAGTC NGG 10 8 17 8 24 0 100 5 10
    240605021541_pov4 TGCTCAGGACTCTGCTGTGGGAGGTGTAGCAG(+ACTCAC)GATCCTGTCTGTACAGAACATGATAGAATAT NGG 10 8 17 8 24 0 100 5 10
    240605021539_2kix GCTTCATATTCTATCATGTTCTGTACAGACAG(+ACTCAC)GATCCTGCTACACCTCCCACAGCAGAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605021537_f4hi TGACTTAGGGAGACAGACCACCACAGACAGGG(+ACTCAC)GATCCTGAGAATCGGGGAGACCCAGGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605021536_nfzo TGCCATCAAGCCTGGGTCTCCCCGATTCTCAG(+ACTCAC)GATCCCCTGTCTGTGGTGGTCTGTCTCCCTA NGG 10 8 17 8 24 0 100 5 10
    240605021534_bd3x ACGTGGGAAAGCGTCACAGGTGGCATCCGAAG(+ACTCAC)GATCCTTCTGCCATGTGTTGTGCATCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240605021533_7mbe CCCTGGCTGAGATGCACAACACATGGCAGAAG(+ACTCAC)GATCCTTCGGATGCCACCTGTGACGCTTTCC NGG 10 8 17 8 24 0 100 5 10