Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605021500_sqz2 GAAGGCAGGGAGGAGTGGCTTAGACAACCGAG(+ACTCAC)GATCCAGTTCCCAGGGTCCAGACGGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605021459_l792 GGTTGAGCCAGCCGTCTGGACCCTGGGAACTG(+ACTCAC)GATCCTCGGTTGTCTAAGCCACTCCTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605021457_4zts GGTCTAATGATGAGCAGATGGTAATGTATGGG(+ACTCAC)GTACCCTTGTGGCTCGCCTTTGGTGGGTACT NGG 10 8 17 8 24 0 100 5 10
    240605021455_4333 GTTTCAGTACCCACCAAAGGCGAGCCACAAGG(+ACTCAC)GTACCCCATACATTACCATCTGCTCATCATT NGG 10 8 17 8 24 0 100 5 10
    240605021454_vnv5 GCCATCGTCTCTACACAGGAGGCCGTTTCTAG(+ACTCAC)GATCCCAGCCTGCTCAGCGAGAGGTCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605021452_ah8i CATTTGCTCTGACCTCTCGCTGAGCAGGCTGG(+ACTCAC)GATCCTAGAAACGGCCTCCTGTGTAGAGACG NGG 10 8 17 8 24 0 100 5 10
    240605021451_jgzz GCATGCAGGCCTTCCAGGAGGAACACACGTGG(+ACTCAC)GTACCTAGAAACCTAGTGAGGAATGGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605021449_304u TCTAAACTTCCCATTCCTCACTAGGTTTCTAG(+ACTCAC)GTACCCACGTGTGTTCCTCCTGGAAGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605021448_cxz6 CCTGTGAAGCAGACTGGGCTTGGTGATGCTTG(+ACTCAC)GTACCCACTGAGAGTCAGCTGAGATGCGCTT NGG 10 8 17 8 24 0 100 5 10
    240605021447_hv3d GCTGCAAGCGCATCTCAGCTGACTCTCAGTGG(+ACTCAC)GTACCAAGCATCACCAAGCCCAGTCTGCTTC NGG 10 8 17 8 24 0 100 5 10