Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605020702_33ia AGGTAGTCTGCCCTCGTAGAAGGGAGCAGAAG(+ACTCAC)GATCCCGTCTGGGAAGCCGTGGGGTTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605020701_ir4k ATGCTTTCTAAGGATGCTCGATAAACTTATTG(+ACTCAC)GATCCCCAGAGAGCACGTTGGCAGAATCATG NGG 10 8 17 8 24 0 100 5 10
    240605020659_wlng TGAAGCATGATTCTGCCAACGTGCTCTCTGGG(+ACTCAC)GATCCAATAAGTTTATCGAGCATCCTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240605020657_qaq5 ACCTGGAATTTGCCTATGCTCAGTCTTGGATG(+ACTCAC)GATCCGGAATAGTTTCATGGAACAGTGATAA NGG 10 8 17 8 24 0 100 5 10
    240605020655_50l5 GGCAATTATCACTGTTCCATGAAACTATTCCG(+ACTCAC)GATCCATCCAAGACTGAGCATAGGCAAATTC NGG 10 8 17 8 24 0 100 5 10
    240605020653_k0vx CAGATCTTCCCACCTCAGGCTCCAAGTGCTGG(+ACTCAC)GATCCCAACCCTAACCCTAAGGGACAGATTT NGG 10 8 17 8 24 0 100 5 10
    240605020652_vthf GACATAAATCTGTCCCTTAGGGTTAGGGTTGG(+ACTCAC)GATCCCAGCACTTGGAGCCTGAGGTGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605020650_q90i GTCTATTTGATGAGAGAATAACTGGGCACCAG(+ACTCAC)GATCCTATTTTGGAAGCTTCACTTATGTACC NGG 10 8 17 8 24 0 100 5 10
    240605020649_q79v CATATGGTACATAAGTGAAGCTTCCAAAATAG(+ACTCAC)GATCCTGGTGCCCAGTTATTCTCTCATCAAA NGG 10 8 17 8 24 0 100 5 10
    240605020647_d91c GCAAGTGCTCATGACAGTTGCGGGGAAGACCG(+ACTCAC)GATCCGGAGGTGTTACAAGCTTTGATAAATG NGG 10 8 17 8 24 0 100 5 10