Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605020736_ffwk CACTAAAAATATGCTAAAAACTGTTAAGAAGG(+ACTCAC)GATCCTATAGAAGACAGAGATGGAACTGATG NGG 10 8 17 8 24 0 100 5 10
    240605020734_xzlu TATTTTAATATGTTATACATCAGTGTATGTAG(+ACTCAC)GATCCAGAGCTTTCCTTCTGAACCTCACTTC NGG 10 8 17 8 24 0 100 5 10
    240605020733_dk8m TTTCTGAAGTGAGGTTCAGAAGGAAAGCTCTG(+ACTCAC)GATCCTACATACACTGATGTATAACATATTA NGG 10 8 17 8 24 0 100 5 10
    240605020732_wtaq AGATCAGGCCCAGTAGTCTCTAAGAGAGTGAG(+ACTCAC)GATCCTGAGGGAGACTATCCAGCTGCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605020730_yrin GATCTGCCCTGCAGCTGGATAGTCTCCCTCAG(+ACTCAC)GATCCTCACTCTCTTAGAGACTACTGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605020729_eg6i GTGTGTGTGTGTGTGTATGTGTTTCATGTGTG(+ACTCAC)GATCCAAGGGTCGGCCAGGAGCTTAAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605020726_yn7e TTACCCTGCCTTAAGCTCCTGGCCGACCCTTG(+ACTCAC)GATCCACACATGAAACACATACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240605020724_5gva ACCCGCAAGCTCCAGAAACGGCCCCACCTGGG(+ACTCAC)GATCCATCCCATTTACAGTTACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605020722_h09h AGTGTCTGGGTTTGGTAACTGTAAATGGGATG(+ACTCAC)GATCCCCAGGTGGGGCCGTTTCTGGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605020720_20rr AGACCACCTGCTAAAAGGCTCAGAGACAGAGG(+ACTCAC)GATCCTGGCTCTTTTATACAACTAAGAAAAA NGG 10 8 17 8 24 0 100 5 10