Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605020836_pvkp TCCCCAGGGGTTAACGTTCATGGTTCTAGAAG(+ACTCAC)GTACCGTGCAAGCATTCCGAGGGAGGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605020833_r4zw ACAAAACCAGTTTTATGGTGGTGGTGGTGGGG(+ACTCAC)GTACCACCACCACATGATTATATATTGATGG NGG 10 8 17 8 24 0 100 5 10
    240605020832_j15z ATGAGCCATCAATATATAATCATGTGGTGGTG(+ACTCAC)GTACCCCCACCACCACCACCATAAAACTGGT NGG 10 8 17 8 24 0 100 5 10
    240605020830_0x03 CGGCTGGTGAATGAAGAGACTAACCTATTTGG(+ACTCAC)GTACCTATCATGTGTAAAGCCTGGATACATT NGG 10 8 17 8 24 0 100 5 10
    240605020829_ny1x ATGTGAATGTATCCAGGCTTTACACATGATAG(+ACTCAC)GTACCCAAATAGGTTAGTCTCTTCATTCACC NGG 10 8 17 8 24 0 100 5 10
    240605020827_mmxm CCCTCTGTGCATCTGCACTACCTGTGTGTCTG(+ACTCAC)GTACCCGTGAAGGTCAGAAGAGGGTGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605020826_5cvs GAGGATCCTTCACCCTCTTCTGACCTTCACGG(+ACTCAC)GTACCAGACACACAGGTAGTGCAGATGCACA NGG 10 8 17 8 24 0 100 5 10
    240605020825_otx6 ATTAGCAGTAACACCTCCCAGATTGAAGTTCG(+ACTCAC)GTACCCTCTGGACTGTTGTAAATCACCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605020823_hkac TCAGGGGCTGGTGATTTACAACAGTCCAGAGG(+ACTCAC)GTACCGAACTTCAATCTGGGAGGTGTTACTG NGG 10 8 17 8 24 0 100 5 10
    240605020820_f2ty GATTCAGGGTGTCTGGTCCATTGTGGGCCCGG(+ACTCAC)GTACCAGAGGTTTTCCACGAAGATCTCTGGC NGG 10 8 17 8 24 0 100 5 10