Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605020918_axqy GGCAGGTAGGTTTGGAATCGGGGTAGGTACAG(+ACTCAC)GTACCTGTCCCTGAGGAATGGACTCATCTCT NGG 10 8 17 8 24 0 100 5 10
    240605020916_d8q9 TGAAAGAGCTCACACTTCCGAGGCGGAAATGG(+ACTCAC)GTACCAAAATGAGAGATCAGCAAGATTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605020915_qum2 ATCTTGGGAAATCTTGCTGATCTCTCATTTTG(+ACTCAC)GTACCCATTTCCGCCTCGGAAGTGTGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605020914_vzzl TTTCTGTAGGAAATGAAATAACTGATTTTGAG(+ACTCAC)GTACCAAATAGTGCAATTGGGTAAGACAGGG NGG 10 8 17 8 24 0 100 5 10
    240605020913_dapl ATAAACCCTGTCTTACCCAATTGCACTATTTG(+ACTCAC)GTACCTCAAAATCAGTTATTTCATTTCCTAC NGG 10 8 17 8 24 0 100 5 10
    240605020911_nt4p GCAGGCATCCTGAGAGGTGCCATGACAACCAG(+ACTCAC)GATCCTGTTCACAGCCGATCAGTGTGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605020910_jbu7 GAAGTCTCTCCACACTGATCGGCTGTGAACAG(+ACTCAC)GATCCTGGTTGTCATGGCACCTCTCAGGATG NGG 10 8 17 8 24 0 100 5 10
    240605020909_8pii TGAATATAAGGGATCTTACTGTCATTTGTTAG(+ACTCAC)GTACCACTGAGTAATTCCATAGTTTCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605020907_4lfe AATTACCACAGAAACTATGGAATTACTCAGTG(+ACTCAC)GTACCTAACAAATGACAGTAAGATCCCTTAT NGG 10 8 17 8 24 0 100 5 10
    240605020905_5u53 TGCCTGCAGTTTTAACTTTATAGCTCTCCCAG(+ACTCAC)GTACCAGCTTTGGGGCCAGCACACGGCACTC NGG 10 8 17 8 24 0 100 5 10