Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605020951_nz6g GAAACAACTGGGAATTTGGACCACTCCATCAG(+ACTCAC)GTACCATCACAGTACCACTCTTCTTCCAAGT NGG 10 8 17 8 24 0 100 5 10
    240605020950_o7e0 TCTGGAGAAACTCACGACAGAGGTGGTGCTTG(+ACTCAC)GTACCTAGTTCCCAGGGCCAGGTAAACTTGT NGG 10 8 17 8 24 0 100 5 10
    240605020949_7dvl GTGACACAAGTTTACCTGGCCCTGGGAACTAG(+ACTCAC)GTACCAAGCACCACCTCTGTCGTGAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605020948_8e76 CCACTGCTGCGACACGTGGAGGCCCCACAGTG(+ACTCAC)GTACCCCAGAGTGGGAAGGTTGGATGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605020946_u9f5 TCCCATGAGCCATCCAACCTTCCCACTCTGGG(+ACTCAC)GTACCACTGTGGGGCCTCCACGTGTCGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605020945_bpm9 GAGGTCCATTCTCACTGGTTGGGGTCCATGTG(+ACTCAC)GTACCAGTTCCCAAGTATTTACTCTACACAG NGG 10 8 17 8 24 0 100 5 10
    240605020944_omkr TCTCTCTGTGTAGAGTAAATACTTGGGAACTG(+ACTCAC)GTACCACATGGACCCCAACCAGTGAGAATGG NGG 10 8 17 8 24 0 100 5 10
    240605020940_v8v0 TCGCTCGATCAGCTTGTTTTTGTACGCCCCAG(+ACTCAC)GATCCTATGCTCAGGGGAGGTGTAGCCCACA NGG 10 8 17 8 24 0 100 5 10
    240605020939_d055 GTGGCTGTGGGCTACACCTCCCCTGAGCATAG(+ACTCAC)GATCCTGGGGCGTACAAAAACAAGCTGATCG NGG 10 8 17 8 24 0 100 5 10
    240605020938_2jlz GTCCTCTGGCTTCTGCTACACCATCAATCCTG(+ACTCAC)GATCCTCACCAGGACTTCTCTCAGTTATCCT NGG 10 8 17 8 24 0 100 5 10