Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605021051_v4db AAAATATATCGGCAGTCACTTCTTTTATAAAG(+ACTCAC)GTACCATGGGCTACATTACACAATAGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605021048_zx2g TACATTGAGACTATTGTGTAATGTAGCCCATG(+ACTCAC)GTACCTTTATAAAAGAAGTGACTGCCGATAT NGG 10 8 17 8 24 0 100 5 10
    240605021047_t9a7 GTTTGATACCCAGGCCTTGCTCTGCTTAGTAG(+ACTCAC)GTACCATGGGCCGTTTTAAGAGCTACACATG NGG 10 8 17 8 24 0 100 5 10
    240605021045_iqnx TCTGCCATGTGTAGCTCTTAAAACGGCCCATG(+ACTCAC)GTACCTACTAAGCAGAGCAAGGCCTGGGTAT NGG 10 8 17 8 24 0 100 5 10
    240605021043_y98v CACTGAGTCAGGGCTCCGGCTTCTGGCTGTGG(+ACTCAC)GTACCCACCGCCCAGCTGCAAGAGGGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605021041_u7ro ATTGGTGACTCCCTCTTGCAGCTGGGCGGTGG(+ACTCAC)GTACCCACAGCCAGAAGCCGGAGCCCTGACT NGG 10 8 17 8 24 0 100 5 10
    240605021040_95mx TTTAAGTCCCCAGGGTAGCTGGGGGCAGTTAG(+ACTCAC)GTACCCCTTGCTCCCCCAGCCCTCCCCACCT NGG 10 8 17 8 24 0 100 5 10
    240605021038_lzde GGCCTAGGTGGGGAGGGCTGGGGGAGCAAGGG(+ACTCAC)GTACCTAACTGCCCCCAGCTACCCTGGGGAC NGG 10 8 17 8 24 0 100 5 10
    240605021037_88fi TTGGGACCCAATTCCCCTTTCTCCAAGACAGG(+ACTCAC)GATCCAGCGGCCCAGGATGCCCACTGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605021035_fqhs AGCAGGACCCCAGTGGGCATCCTGGGCCGCTG(+ACTCAC)GATCCCTGTCTTGGAGAAAGGGGAATTGGGT NGG 10 8 17 8 24 0 100 5 10