Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605021106_stuc TTGGTGTTTCCTGACCTGATCTGCAAACTGGG(+ACTCAC)GATCCCTGGTCTGTACCTGAATTCTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605021104_g9n2 ATATGAAAGAAAGAATTCAGGTACAGACCAGG(+ACTCAC)GATCCCCAGTTTGCAGATCAGGTCAGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240605021103_xhvi CCCTCACCCCAGAGTAAGCTGCACTGTTCTTG(+ACTCAC)GTACCAGAGATCCGCTAGTGCACAAAGACTC NGG 10 8 17 8 24 0 100 5 10
    240605021102_7alp TTACCGAGTCTTTGTGCACTAGCGGATCTCTG(+ACTCAC)GTACCAAGAACAGTGCAGCTTACTCTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240605021101_99x0 TGCAGCCGCAGCACCCGAGTCTGGAGAGAGAG(+ACTCAC)GATCCCTTGAGCACAGGGATTGGAGGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605021059_ovni CCCAGGCTGGCCTCCAATCCCTGTGCTCAAGG(+ACTCAC)GATCCTCTCTCTCCAGACTCGGGTGCTGCGG NGG 10 8 17 8 24 0 100 5 10
    240605021057_xjr9 TGTGACTGAGCTAGCCAGCCAGTAAGTTCAAG(+ACTCAC)GATCCTCCTGGCCTCGCGCCCCAACACTGTG NGG 10 8 17 8 24 0 100 5 10
    240605021056_67b1 GGGACCACAGTGTTGGGGCGCGAGGCCAGGAG(+ACTCAC)GATCCTTGAACTTACTGGCTGGCTAGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240605021054_20au ATCTTCATTCCTGAGCCACCTTGCCTCCTCTG(+ACTCAC)GATCCCTTTTCTAAGAAAACACACTTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605021052_ytiq AGTGCAGACCAAGTGTGTTTTCTTAGAAAAGG(+ACTCAC)GATCCAGAGGAGGCAAGGTGGCTCAGGAATG NGG 10 8 17 8 24 0 100 5 10