Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605021151_uqey CTGAGCCTGTGGTCAGCTGACCACAGAGGTGG(+ACTCAC)GTACCTGAGTCCCCATGGCCCCCTGGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240605021150_uxp5 ATAGGACCTTCCAGGGGGCCATGGGGACTCAG(+ACTCAC)GTACCCACCTCTGTGGTCAGCTGACCACAGG NGG 10 8 17 8 24 0 100 5 10
    240605021149_foft AGGCCTAGGACCAAGGTATCCTCTGTCTGCAG(+ACTCAC)GTACCTGACTACCCACAACAGGGAGGCTCGC NGG 10 8 17 8 24 0 100 5 10
    240605021148_t9y6 AGATGGCGAGCCTCCCTGTTGTGGGTAGTCAG(+ACTCAC)GTACCTGCAGACAGAGGATACCTTGGTCCTA NGG 10 8 17 8 24 0 100 5 10
    240605021147_bvgx TCACCAAGAGGTCCCCACGATAGGCGAGACGG(+ACTCAC)GATCCTGATGGCCCTGAGCCCGGCAGGGTCA NGG 10 8 17 8 24 0 100 5 10
    240605021145_omr8 ACGGCTGACCCTGCCGGGCTCAGGGCCATCAG(+ACTCAC)GATCCCGTCTCGCCTATCGTGGGGACCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605021143_nts1 TCTTCACAATAGCACCCTTCCCAGCAGCCAGG(+ACTCAC)GATCCTGGTATCCTGGCTAAACCCATTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605021141_o01m AGGCCAGGGAATGGGTTTAGCCAGGATACCAG(+ACTCAC)GATCCCTGGCTGCTGGGAAGGGTGCTATTGT NGG 10 8 17 8 24 0 100 5 10
    240605021140_oh7n CCTGAAGGATTCTCCCTAATTCCTTTCCTTCG(+ACTCAC)GATCCTCCTCAGGCTCCAGCCACAGTAAATG NGG 10 8 17 8 24 0 100 5 10
    240605021138_bvjg AAATACATTTACTGTGGCTGGAGCCTGAGGAG(+ACTCAC)GATCCGAAGGAAAGGAATTAGGGAGAATCCT NGG 10 8 17 8 24 0 100 5 10