Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605021240_4keg GGCTAAGTTGAAACAGCCTGTATTCCCTCAAG(+ACTCAC)GATCCCTACTCTTGGCCCTCTGACCTAATGA NGG 10 8 17 8 24 0 100 5 10
    240605021238_dud0 ATACCTCATTAGGTCAGAGGGCCAAGAGTAGG(+ACTCAC)GATCCTTGAGGGAATACAGGCTGTTTCAACT NGG 10 8 17 8 24 0 100 5 10
    240605021237_ipdj TGTCTCTTAAGTGCTAGGATTTAAGGTGTGTG(+ACTCAC)GTACCACATCTGGATGGCATTTGGCTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605021236_m6bp GAAGTTAAAAAGCCAAATGCCATCCAGATGTG(+ACTCAC)GTACCACACACCTTAAATCCTAGCACTTAAG NGG 10 8 17 8 24 0 100 5 10
    240605021235_2yj4 AAGGGCCAGAGCTGGGGGGCAAGAAGCTTGTG(+ACTCAC)GATCCTTCCCCCCATGCACTGTGAAGGACCC NGG 10 8 17 8 24 0 100 5 10
    240605021233_bmfh AACGAGGGTCCTTCACAGTGCATGGGGGGAAG(+ACTCAC)GATCCACAAGCTTCTTGCCCCCCAGCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605021231_d9pj CATTCTTTTCACCAGCACCCATCGCCCAAGGG(+ACTCAC)GTACCAAGGGGGGCAAGGGGTGTCCAGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605021229_1svp GGGCTTGGACTGGACACCCCTTGCCCCCCTTG(+ACTCAC)GTACCCCTTGGGCGATGGGTGCTGGTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605021227_jqoj CCATCCTCCCGCAGGAGGAGGGGAACAGGCGG(+ACTCAC)GATCCTGGGTCCCTCGTAAGCACTTTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605021225_ozyu CGGTAAAACCAAAGTGCTTACGAGGGACCCAG(+ACTCAC)GATCCCGCCTGTTCCCCTCCTCCTGCGGGAG NGG 10 8 17 8 24 0 100 5 10