Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605021310_ug61 CAGATCTGCAATTCTCTGTTCTCTCGGCCTGG(+ACTCAC)GATCCCAGCTCTCAGCAATCAAAATTGTTGC NGG 10 8 17 8 24 0 100 5 10
    240605021309_4mlg TTGATGCAACAATTTTGATTGCTGAGAGCTGG(+ACTCAC)GATCCCAGGCCGAGAGAACAGAGAATTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605021308_stem TGGCACAACTGCCTGCTCCTCTAGTACTAGAG(+ACTCAC)GATCCAATGCCCTCAAATTCTCAAGATACTT NGG 10 8 17 8 24 0 100 5 10
    240605021306_4gqh TTTTTAAGTATCTTGAGAATTTGAGGGCATTG(+ACTCAC)GATCCTCTAGTACTAGAGGAGCAGGCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605021305_g2p2 CTCCACTCATAATCATGTCTACAGAGCTCTAG(+ACTCAC)GTACCTTTTGACAGGTGGAATAAAGTCTCAA NGG 10 8 17 8 24 0 100 5 10
    240605021304_0263 AAGATTTGAGACTTTATTCCACCTGTCAAAAG(+ACTCAC)GTACCTAGAGCTCTGTAGACATGATTATGAG NGG 10 8 17 8 24 0 100 5 10
    240605021303_25fn GTGTCCTGGATTTCCTGGATGTTTTGAGTTAG(+ACTCAC)GATCCTTTTGCATTTTGTATTTTCTTTGACT NGG 10 8 17 8 24 0 100 5 10
    240605021302_4o7l ACAACAGTCAAAGAAAATACAAAATGCAAAAG(+ACTCAC)GATCCTAACTCAAAACATCCAGGAAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240605021300_08k9 AGGAGTTGTGTTCCACTCACCAGAGGTCTTAG(+ACTCAC)GATCCCGTGGGGAATCCTGTGTGGGCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605021258_noei ACCCGCACAGGCCCACACAGGATTCCCCACGG(+ACTCAC)GATCCTAAGACCTCTGGTGAGTGGAACACAA NGG 10 8 17 8 24 0 100 5 10