Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605021208_0b3m TTTGTGCCTTCTGTGAGGGATAGGGGACAAGG(+ACTCAC)GATCCCAGGTGTCCCCATTTCCCGCCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605021206_fe30 AAGGTGAGGGGGCGGGAAATGGGGACACCTGG(+ACTCAC)GATCCCTTGTCCCCTATCCCTCACAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605021204_79rz TGTGTCAGAGGTAGAGATGGCACCTCTGGAGG(+ACTCAC)GTACCAGAGCTAGGTGGATACATGGACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605021203_bs3t TGCCCCTGGGTCCATGTATCCACCTAGCTCTG(+ACTCAC)GTACCCTCCAGAGGTGCCATCTCTACCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605021202_p91n CTCTTCAAAATCCAAAACCTGTGGCCTTACTG(+ACTCAC)GTACCAAATTCTCCTTGTAAACATACCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605021200_drmx CTGTAGCCAGGTATGTTTACAAGGAGAATTTG(+ACTCAC)GTACCAGTAAGGCCACAGGTTTTGGATTTTG NGG 10 8 17 8 24 0 100 5 10
    240605021158_afgm GCTGAGGCAGGCACACTATCCTCACTCCCTCG(+ACTCAC)GTACCTGATCTCTAAATCACTTCTCAACTGA NGG 10 8 17 8 24 0 100 5 10
    240605021157_1okh TGGGATCAGTTGAGAAGTGATTTAGAGATCAG(+ACTCAC)GTACCGAGGGAGTGAGGATAGTGTGCCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605021154_16iw CTGGACTACATATAAATTCAGATCAGCCAAGG(+ACTCAC)GTACCTAGAAAGACCTATTTCAAAACAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605021153_g82e CTTGTTTTTTGTTTTGAAATAGGTCTTTCTAG(+ACTCAC)GTACCCTTGGCTGATCTGAATTTATATGTAG NGG 10 8 17 8 24 0 100 5 10