Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605021006_5ero CCTGTGTATGCTGTAATAGAGGGCAGGGCTGG(+ACTCAC)GATCCCAGCACTGACTCTGCCCTTCATCTGC NGG 10 8 17 8 24 0 100 5 10
    240605021004_ghe9 TATCAGGGCTGGACAATGCCGTAGGAGGAAAG(+ACTCAC)GATCCCCAGAGCAGGTGAACGAGTCAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240605021002_8tnc GGGCTGTCTCTGACTCGTTCACCTGCTCTGGG(+ACTCAC)GATCCTTTCCTCCTACGGCATTGTCCAGCCC NGG 10 8 17 8 24 0 100 5 10
    240605021001_r0jp GCTGTCTTCAGACACACCAGAAGAGGGCATCG(+ACTCAC)GATCCCACTAGATGGTTGTGAGCCATCATGT NGG 10 8 17 8 24 0 100 5 10
    240605020959_ao7b CAACCACATGATGGCTCACAACCATCTAGTGG(+ACTCAC)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605020958_2xvj GACCACCACACCCCGGCGGAAAAGCTGTCTTG(+ACTCAC)GATCCCTTTCTAGGAGTTACAGTGGCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605020957_a6a7 CCTGGAGACAGCCACTGTAACTCCTAGAAAGG(+ACTCAC)GATCCAAGACAGCTTTTCCGCCGGGGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605020955_3oyx GCATGGTGTGCTTTCTGACTGGGGTAGTGTGG(+ACTCAC)GATCCCTCTCTGAGGACCCCGATGTTGGTAC NGG 10 8 17 8 24 0 100 5 10
    240605020953_bvau CCCTAGTACCAACATCGGGGTCCTCAGAGAGG(+ACTCAC)GATCCCACACTACCCCAGTCAGAAAGCACAC NGG 10 8 17 8 24 0 100 5 10
    240605020952_2voy TGGAAACTTGGAAGAAGAGTGGTACTGTGATG(+ACTCAC)GTACCTGATGGAGTGGTCCAAATTCCCAGTT NGG 10 8 17 8 24 0 100 5 10