Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015820_u755 AGGCCAGTCTGGTAGGCAGTTTTTCAATTGAG(+ACTCAC)GATCCCTCTTCTCAGATTACTCTAGCTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605015818_tmmn TTTGATACAAGCTAGAGTAATCTGAGAAGAGG(+ACTCAC)GATCCTCAATTGAAAAACTGCCTACCAGACT NGG 10 8 17 8 24 0 100 5 10
    240605015817_my0q TAATCCCAGCTCCTAGGAGGCAGGGACAAGTG(+ACTCAC)GATCCCTGTGAGTTAGAGACTAGCCTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605015816_wezz TATGTAGACCAGGCTAGTCTCTAACTCACAGG(+ACTCAC)GATCCACTTGTCCCTGCCTCCTAGGAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605015815_td26 GAAGAGAGACAAGGAGAAGCAGAAAGCAGAAG(+ACTCAC)GTACCCTTTTATTGGCATTTTAACAATGGCA NGG 10 8 17 8 24 0 100 5 10
    240605015813_zprn CGTGCTGCCATTGTTAAAATGCCAATAAAAGG(+ACTCAC)GTACCTTCTGCTTTCTGCTTCTCCTTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605015811_09w9 TCTTTTTGTAAGGTTCTGAGACTTTATGGAAG(+ACTCAC)GTACCTCTGCATTCTCATAAACCCTGACTCT NGG 10 8 17 8 24 0 100 5 10
    240605015810_b0d4 GGATCAGAGTCAGGGTTTATGAGAATGCAGAG(+ACTCAC)GTACCTTCCATAAAGTCTCAGAACCTTACAA NGG 10 8 17 8 24 0 100 5 10
    240605015809_awyn AACAGCCGTGGGCGGTGGGTGTTGTTACTGTG(+ACTCAC)GTACCTACCATGCCATCTTAACCGAAACCAA NGG 10 8 17 8 24 0 100 5 10
    240605015807_nj86 AGGTCTTGGTTTCGGTTAAGATGGCATGGTAG(+ACTCAC)GTACCACAGTAACAACACCCACCGCCCACGG NGG 10 8 17 8 24 0 100 5 10