Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015734_rz0c ATGTCCCAACCAGCACCTTGGCCACATTCCTG(+ACTCAC)GATCCTATGCTTGTCCCAGAGGAGAAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605015733_i9jg CTCTGCAGCCTTCTCCTCTGGGACAAGCATAG(+ACTCAC)GATCCAGGAATGTGGCCAAGGTGCTGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240605015731_ir5k AGAATCGCTTAAGCAGGGCTGTGGGCCTGCAG(+ACTCAC)GATCCCCAGGTCCCTTTGGACCGGAGGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605015729_psic CGTCTTCCCCCTCCGGTCCAAAGGGACCTGGG(+ACTCAC)GATCCTGCAGGCCCACAGCCCTGCTTAAGCG NGG 10 8 17 8 24 0 100 5 10
    240605015728_jxd3 TTGTGGGTCATGAATTCTCTGTACCCCTGCTG(+ACTCAC)GATCCTTCTAATTAAATTTGTTTTTCCTTCG NGG 10 8 17 8 24 0 100 5 10
    240605015726_z7ng GGTGACGAAGGAAAAACAAATTTAATTAGAAG(+ACTCAC)GATCCAGCAGGGGTACAGAGAATTCATGACC NGG 10 8 17 8 24 0 100 5 10
    240605015724_rjtq CTGTGAAGTCTATTGTGGCCACAGACTGCTGG(+ACTCAC)GTACCAAGTCTCAGGAGAACCCAGCCTAGAT NGG 10 8 17 8 24 0 100 5 10
    240605015723_4z0p CCTAAATCTAGGCTGGGTTCTCCTGAGACTTG(+ACTCAC)GTACCCAGCAGTCTGTGGCCACAATAGACTT NGG 10 8 17 8 24 0 100 5 10
    240605015722_1dmd GATAATCTATCATTTGAATGGCAAGAGACCTG(+ACTCAC)GTACCCTCTATCTCGCTACTCTGGAGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240605015720_63yu CTAGGGGAGGCTCCAGAGTAGCGAGATAGAGG(+ACTCAC)GTACCAGGTCTCTTGCCATTCAAATGATAGA NGG 10 8 17 8 24 0 100 5 10