Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015627_f2on TTATCATCTCTGACCTTCTGAAGCCAGGGGTG(+ACTCAC)GTACCCATCCCTAGCTCAGTCTAATTAAATC NGG 10 8 17 8 24 0 100 5 10
    240605015626_gh2b GGAAAGATTTAATTAGACTGAGCTAGGGATGG(+ACTCAC)GTACCACCCCTGGCTTCAGAAGGTCAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240605015625_i9br TGTACTTTGGGCAGGTGATGAAATTTACACAG(+ACTCAC)GTACCTCAAGTCAAGACCTGCGCGTGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605015623_002h CCAAATGCAGCACGCGCAGGTCTTGACTTGAG(+ACTCAC)GTACCTGTGTAAATTTCATCACCTGCCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605015622_n1me TCTAGTCCCTTCTCCACATCACCTAAGGGTGG(+ACTCAC)GTACCTCCATTGCGATGTTGTGGCATCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605015620_purp AAAAGTTAAGATGCCACAACATCGCAATGGAG(+ACTCAC)GTACCCACCCTTAGGTGATGTGGAGAAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605015618_3imt CAAGCAGCCATCTAACTGCTAGAGTATACTGG(+ACTCAC)GATCCTAACCTACCCTCTTGGGAAACGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605015617_k853 TTCCCCCTCCGTTTCCCAAGAGGGTAGGTTAG(+ACTCAC)GATCCCAGTATACTCTAGCAGTTAGATGGCT NGG 10 8 17 8 24 0 100 5 10
    240605015616_12vf GTCGCCACCCCCGCCTTTGGAATGTGGGAATG(+ACTCAC)GATCCATGAGGACACGCATGGTCACTCACTT NGG 10 8 17 8 24 0 100 5 10
    240605015615_nr5i TCAGTAAGTGAGTGACCATGCGTGTCCTCATG(+ACTCAC)GATCCATTCCCACATTCCAAAGGCGGGGGTG NGG 10 8 17 8 24 0 100 5 10