Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015644_2avv ATCAGCTGCAGCCTGGCTGCCTTATCTCCCTG(+ACTCAC)GTACCACTGGCTTCTGCAACCCATGGGGTTA NGG 10 8 17 8 24 0 100 5 10
    240605015642_6tbu GCCATTAACCCCATGGGTTGCAGAAGCCAGTG(+ACTCAC)GTACCAGGGAGATAAGGCAGCCAGGCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605015640_jmgc GGACTTGCAGACCCAGCACTCACGGGCACTGG(+ACTCAC)GATCCATCCCAGAGAGTGCACCTGAGTAGCT NGG 10 8 17 8 24 0 100 5 10
    240605015638_w6yh GGAAGAGCTACTCAGGTGCACTCTCTGGGATG(+ACTCAC)GATCCCAGTGCCCGTGAGTGCTGGGTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605015636_i87z CGGACATGGAATCTTGAAGACCCAAGGCAGGG(+ACTCAC)GATCCCAAGGGCTCCATGCAGAACTCACAGG NGG 10 8 17 8 24 0 100 5 10
    240605015634_ercg ATCCTCCTGTGAGTTCTGCATGGAGCCCTTGG(+ACTCAC)GATCCCCTGCCTTGGGTCTTCAAGATTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605015633_ydcr TTATTTTTCTAAATAGTGTTCAGAAAACTCTG(+ACTCAC)GATCCAAAATGTGGCATTTTTCTGAGAATGA NGG 10 8 17 8 24 0 100 5 10
    240605015631_rbjc AATCTTCATTCTCAGAAAAATGCCACATTTTG(+ACTCAC)GATCCAGAGTTTTCTGAACACTATTTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605015630_o8j4 ACTAAGCTGATCTGGCTATCCCAATGCATGTG(+ACTCAC)GTACCTGATAGATGGATATGGTAGAGATTAT NGG 10 8 17 8 24 0 100 5 10
    240605015629_i6rz GGGAAATAATCTCTACCATATCCATCTATCAG(+ACTCAC)GTACCACATGCATTGGGATAGCCAGATCAGC NGG 10 8 17 8 24 0 100 5 10