Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015700_7g3s CTAAACTTCTAGCCCAACATGCATGTGCCCTG(+ACTCAC)GATCCAACTTCTAGCCCAACACAACTGAACA NGG 10 8 17 8 24 0 100 5 10
    240605015659_mknb CGTTTTGTTCAGTTGTGTTGGGCTAGAAGTTG(+ACTCAC)GATCCAGGGCACATGCATGTTGGGCTAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605015657_wd5c TTAGTCTGGTACAATCCTTTCTAGAAGAAAGG(+ACTCAC)GATCCCTAAAGTGAAGGAATAGATTCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605015656_6bhe GATGGGAGGGGAATCTATTCCTTCACTTTAGG(+ACTCAC)GATCCCTTTCTTCTAGAAAGGATTGTACCAG NGG 10 8 17 8 24 0 100 5 10
    240605015654_y7f3 GCTTCCTGAACCTCACTCTCACTTCCTAGGGG(+ACTCAC)GATCCTGGAAGCTGGGTACGAACTAGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605015652_igfe GTCTCCTTCCCTAGTTCGTACCCAGCTTCCAG(+ACTCAC)GATCCCCCTAGGAAGTGAGAGTGAGGTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605015650_pt0w TGCACTCAGGAGAGGGAGAACCTCCCTGGATG(+ACTCAC)GTACCCAGATGAAGTCAGAGCCGTGCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605015649_8d2y GGCAACAAGAGCACGGCTCTGACTTCATCTGG(+ACTCAC)GTACCATCCAGGGAGGTTCTCCCTCTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605015647_mabj CTGGGAAGCAGAGGCAAGCTCAGGATACAAGG(+ACTCAC)GTACCTTCCCCTTGCCTGGGTCTTCCCTCGA NGG 10 8 17 8 24 0 100 5 10
    240605015646_8kf2 TAGGCTCGAGGGAAGACCCAGGCAAGGGGAAG(+ACTCAC)GTACCCTTGTATCCTGAGCTTGCCTCTGCTT NGG 10 8 17 8 24 0 100 5 10