Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015459_9mmn CTAGGTGTTCAGGGACCTCCAAAAGCTCCAAG(+ACTCAC)GATCCACCTCTTGCTACATCTTCAGTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605015458_kfna CTCTATTCCTACTGAAGATGTAGCAAGAGGTG(+ACTCAC)GATCCTTGGAGCTTTTGGAGGTCCCTGAACA NGG 10 8 17 8 24 0 100 5 10
    240605015456_3kz7 TTGAGCGAGCGAGAGATTATTCTAATGTCCTG(+ACTCAC)GTACCCGGCTGGCTTCTGGGAGGACTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605015454_x8jo TCGAAAAGCCAGTCCTCCCAGAAGCCAGCCGG(+ACTCAC)GTACCAGGACATTAGAATAATCTCTCGCTCG NGG 10 8 17 8 24 0 100 5 10
    240605015452_d1rd AAGCTTATTGGCCAAACCCTCAGGGCCTCTAG(+ACTCAC)GTACCTCATTAGCATGGAGAACTCTGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605015451_mf9n ATCATCAGAACAGAGTTCTCCATGCTAATGAG(+ACTCAC)GTACCTAGAGGCCCTGAGGGTTTGGCCAATA NGG 10 8 17 8 24 0 100 5 10
    240605015450_dc10 TATGTGGGTGTGAGGTGGTCAGGCCATTTGTG(+ACTCAC)GTACCCGTAGTGTTTGGTGCTGGCCCTAGCC NGG 10 8 17 8 24 0 100 5 10
    240605015449_8gyf ATAGTGGCTAGGGCCAGCACCAAACACTACGG(+ACTCAC)GTACCACAAATGGCCTGACCACCTCACACCC NGG 10 8 17 8 24 0 100 5 10
    240605015448_glf6 TGGAAGCAAGGATGAACGCTTTCCTAGGTCTG(+ACTCAC)GTACCGTTTCCTCTCAATGGCCTCACCATAC NGG 10 8 17 8 24 0 100 5 10
    240605015446_lm6v ACTGAGTATGGTGAGGCCATTGAGAGGAAACG(+ACTCAC)GTACCAGACCTAGGAAAGCGTTCATCCTTGC NGG 10 8 17 8 24 0 100 5 10