Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015358_idwt CCATCTTTCTGTACACTAGATTTCTTTCTTTG(+ACTCAC)GATCCTTAAAGATTTCTTTCCAGTGTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605015357_zg1b TAAAACCTTTACACTGGAAAGAAATCTTTAAG(+ACTCAC)GATCCAAAGAAAGAAATCTAGTGTACAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605015356_2myt CCCATCCACCTGGCATTTATTTGGCTTCTAGG(+ACTCAC)GATCCAAACTCCGGCTCTCTTCACGCATGGC NGG 10 8 17 8 24 0 100 5 10
    240605015354_2ruv TACTTGCCATGCGTGAAGAGAGCCGGAGTTTG(+ACTCAC)GATCCCTAGAAGCCAAATAAATGCCAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605015353_btzs GTGTGGAGCTTGGCAGCCTCTACCTGGAGTGG(+ACTCAC)GTACCATCTGCCTGACGTTGCAACTGATAGC NGG 10 8 17 8 24 0 100 5 10
    240605015351_cb1q CCGTGGCTATCAGTTGCAACGTCAGGCAGATG(+ACTCAC)GTACCCACTCCAGGTAGAGGCTGCCAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605015350_y5aa GTGGTTAGGGTGTATAAAACCTGAACTTGCAG(+ACTCAC)GATCCGGTGAAGCCTTTATTTAGCATGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605015348_v6lr GTAGCCACACATGCTAAATAAAGGCTTCACCG(+ACTCAC)GATCCTGCAAGTTCAGGTTTTATACACCCTA NGG 10 8 17 8 24 0 100 5 10
    240605015347_3k16 CAACCCACAAGCAGGTGTGTGAACCAGAATGG(+ACTCAC)GATCCGCTGATGGCTGGCATGCCTTGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605015345_dpy4 TAGCCTCTCCCAAGGCATGCCAGCCATCAGCG(+ACTCAC)GATCCCATTCTGGTTCACACACCTGCTTGTG NGG 10 8 17 8 24 0 100 5 10