Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015311_kjfr CTCTGCAGGCAAGCTCTCTTCTTGCAGGGCAG(+ACTCAC)GTACCCAGATATCTGGTGTTCGAACCAGACT NGG 10 8 17 8 24 0 100 5 10
    240605015310_hezx CCAGGAGTCTGGTTCGAACACCAGATATCTGG(+ACTCAC)GTACCTGCCCTGCAAGAAGAGAGCTTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605015308_2eis CTTTGGGCTGTCCTCCGTGAGTGTCATCCATG(+ACTCAC)GTACCTGCTCTAGGAATTCATTTCTCCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605015307_omfb CTGGAGTCTGGAGAAATGAATTCCTAGAGCAG(+ACTCAC)GTACCATGGATGACACTCACGGAGGACAGCC NGG 10 8 17 8 24 0 100 5 10
    240605015305_rrxf CCTCAAACTATTCCACAAAATAGAAACAGAAG(+ACTCAC)GTACCCTATCCAATTCATTCTATGAAACCAC NGG 10 8 17 8 24 0 100 5 10
    240605015304_ugt5 TAATTGTGGTTTCATAGAATGAATTGGATAGG(+ACTCAC)GTACCTTCTGTTTCTATTTTGTGGAATAGTT NGG 10 8 17 8 24 0 100 5 10
    240605015302_axj3 CCTGCTGTGACTAGATGTCTCAGAGCAGGATG(+ACTCAC)GTACCCAACGGGGCCTCCCCTGAGGAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605015300_nkpd CCTCCCCTTCTCCTCAGGGGAGGCCCCGTTGG(+ACTCAC)GTACCATCCTGCTCTGAGACATCTAGTCACA NGG 10 8 17 8 24 0 100 5 10
    240605015259_69g1 GCTGATTACATTCCTCAGAAAGACCACAGTGG(+ACTCAC)GTACCGACCATTGGCCACCTGTGGATAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605015257_cmol GGCTTCTCTTATCCACAGGTGGCCAATGGTCG(+ACTCAC)GTACCCACTGTGGTCTTTCTGAGGAATGTAA NGG 10 8 17 8 24 0 100 5 10