Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015208_ore9 TCAAAGACCTCAAGAACTGCCTATGGACCCAG(+ACTCAC)GTACCTTCCCAATGCATGAGCTACAAACCCA NGG 10 8 17 8 24 0 100 5 10
    240605015206_mvip GAAGGTGGGTTTGTAGCTCATGCATTGGGAAG(+ACTCAC)GTACCTGGGTCCATAGGCAGTTCTTGAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605015203_h2i2 CACCAAGCCTGATATCTGTGTTTGAACCCTAG(+ACTCAC)GATCCATATGGTAGGAGAGAACTAACCCACA NGG 10 8 17 8 24 0 100 5 10
    240605015202_72xv CAACTTGTGGGTTAGTTCTCTCCTACCATATG(+ACTCAC)GATCCTAGGGTTCAAACACAGATATCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605015200_momm TGGGTTTGGACTCCTTGTCTACATCTGTCCCG(+ACTCAC)GATCCTCAACGCTCTCCAAAGGACAGCCTAC NGG 10 8 17 8 24 0 100 5 10
    240605015159_qnpr CGGTGGTAGGCTGTCCTTTGGAGAGCGTTGAG(+ACTCAC)GATCCGGGACAGATGTAGACAAGGAGTCCAA NGG 10 8 17 8 24 0 100 5 10
    240605015157_ybqy TATATTTAAAAAACAAAAACAGTCTTGCCATG(+ACTCAC)GATCCAGTGTTAACACTGTCATTTTGTAACA NGG 10 8 17 8 24 0 100 5 10
    240605015155_0lue AAGGCTGTTACAAAATGACAGTGTTAACACTG(+ACTCAC)GATCCATGGCAAGACTGTTTTTGTTTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605015154_wsqf AGACCCCACACTTTTTGTAAGACATCAATCAG(+ACTCAC)GATCCCCATATTAATTATAAGCATCACTGAA NGG 10 8 17 8 24 0 100 5 10
    240605015152_g2zd CAACATTCAGTGATGCTTATAATTAATATGGG(+ACTCAC)GATCCTGATTGATGTCTTACAAAAAGTGTGG NGG 10 8 17 8 24 0 100 5 10