Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015123_rsr6 CTTCACACAACATCTGCATTCACATATTTTAG(+ACTCAC)GATCCTTTGTCTCTCTTCCTGTACACTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605015122_pypj AGATGAAAAAGTGTACAGGAAGAGAGACAAAG(+ACTCAC)GATCCTAAAATATGTGAATGCAGATGTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605015120_81xe TGGTCCAAAAGTCAATGGCTGCAGTCCGTGTG(+ACTCAC)GATCCAATGGTAGAGACTTTTTTCTAGTACA NGG 10 8 17 8 24 0 100 5 10
    240605015118_feap AAAAGTGTACTAGAAAAAAGTCTCTACCATTG(+ACTCAC)GATCCACACGGACTGCAGCCATTGACTTTTG NGG 10 8 17 8 24 0 100 5 10
    240605015117_2z7p TGCTCCTACAAGGCTCACAGAAGAAGAGCATG(+ACTCAC)GATCCCCTTCAGCAAGAATGCTTAAGACTCG NGG 10 8 17 8 24 0 100 5 10
    240605015115_9o1v TGACACGAGTCTTAAGCATTCTTGCTGAAGGG(+ACTCAC)GATCCATGCTCTTCTTCTGTGAGCCTTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605015114_j46c AAAGGAAAGAGGAAAAAAAAAGGTCAAAAAAG(+ACTCAC)GTACCAAAAGTCACACACAAAAAACACAAGA NGG 10 8 17 8 24 0 100 5 10
    240605015113_rjnc AATACTCTTGTGTTTTTTGTGTGTGACTTTTG(+ACTCAC)GTACCTTTTTTGACCTTTTTTTTTCCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605015112_d5c3 AATAAAGTCAGATCATTACAATCTTAACTGTG(+ACTCAC)GTACCCTTTTTTGATCAAAAAAACCTAGTTA NGG 10 8 17 8 24 0 100 5 10
    240605015110_a8yf AACAGTAACTAGGTTTTTTTGATCAAAAAAGG(+ACTCAC)GTACCACAGTTAAGATTGTAATGATCTGACT NGG 10 8 17 8 24 0 100 5 10