Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015054_7q15 GGTTATAGTTTCAGTGTAGCTCTGGGAACAGG(+ACTCAC)GATCCCTGGACCACTAATACTTTCTAGATCT NGG 10 8 17 8 24 0 100 5 10
    240605015052_xcx3 TAGATAGATCTAGAAAGTATTAGTGGTCCAGG(+ACTCAC)GATCCCTGTTCCCAGAGCTACACTGAAACTA NGG 10 8 17 8 24 0 100 5 10
    240605015051_031s AATTTTCCATTCCTGCATTAGTCTAGTGTATG(+ACTCAC)GATCCACGTGCTCTCTTTTAGTATTATCCTA NGG 10 8 17 8 24 0 100 5 10
    240605015049_6hiq ACTTATAGGATAATACTAAAAGAGAGCACGTG(+ACTCAC)GATCCATACACTAGACTAATGCAGGAATGGA NGG 10 8 17 8 24 0 100 5 10
    240605015048_ad9b TAAATTCAATATAAATTGTGATCTCTTGCTGG(+ACTCAC)GATCCCAGGCCCATTGTGTTTCCAGGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605015046_pt0l TCCCTACAGCCCTGGAAACACAATGGGCCTGG(+ACTCAC)GATCCCAGCAAGAGATCACAATTTATATTGA NGG 10 8 17 8 24 0 100 5 10
    240605015044_x84z TAGGTAACAAATTTATCAATCAACTATGTTTG(+ACTCAC)GATCCAGGGAATCTCAAGTTATTCAATTCAT NGG 10 8 17 8 24 0 100 5 10
    240605015042_678p AGAGAATGAATTGAATAACTTGAGATTCCCTG(+ACTCAC)GATCCAAACATAGTTGATTGATAAATTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605015041_gt5v TAACTACACTTCTAAGGCAGATGCTTCAGATG(+ACTCAC)GTACCCAATAAAGCCTATCAGAAAAGAGGAT NGG 10 8 17 8 24 0 100 5 10
    240605015039_16e9 AGAGCATCCTCTTTTCTGATAGGCTTTATTGG(+ACTCAC)GTACCATCTGAAGCATCTGCCTTAGAAGTGT NGG 10 8 17 8 24 0 100 5 10