Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015136_ohk1 GTTTCAAGATTTTCTAACACAAATTTACATTG(+ACTCAC)GTACCAAAATGGGCAAGAAAATGAAGGCACA NGG 10 8 17 8 24 0 100 5 10
    240605015135_2q65 GAGCCTGTGCCTTCATTTTCTTGCCCATTTTG(+ACTCAC)GTACCAATGTAAATTTGTGTTAGAAAATCTT NGG 10 8 17 8 24 0 100 5 10
    240605015133_z0jv CCAAAAAATACAGCAAAGACAGGAACGTCCAG(+ACTCAC)GATCCGGGTCTGTTAATAAAGCCACCTCGAG NGG 10 8 17 8 24 0 100 5 10
    240605015131_bv7e CTAGTCTCGAGGTGGCTTTATTAACAGACCCG(+ACTCAC)GATCCTGGACGTTCCTGTCTTTGCTGTATTT NGG 10 8 17 8 24 0 100 5 10
    240605015130_er2y ATCAGGCTGGGGAGGTTAGTGCACAATCAGTG(+ACTCAC)GATCCATGCCCCGCCTCCAAGCTCTGCAAAC NGG 10 8 17 8 24 0 100 5 10
    240605015129_tf6p GTGGGGTTTGCAGAGCTTGGAGGCGGGGCATG(+ACTCAC)GATCCACTGATTGTGCACTAACCTCCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605015127_98ca CAGAAGTCAGAGTCATCAGTCATTTGGCTAAG(+ACTCAC)GTACCGCTGAGACACAATATCTCTGTAACTT NGG 10 8 17 8 24 0 100 5 10
    240605015126_ecgt AAAGAAAGTTACAGAGATATTGTGTCTCAGCG(+ACTCAC)GTACCTTAGCCAAATGACTGATGACTCTGAC NGG 10 8 17 8 24 0 100 5 10
    240605015125_sqe1 GGAAAAAACTGAAACACTAAGTGTAGGAAGAG(+ACTCAC)GATCCTAGAAAACTAAACGTATTGACTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605015124_6vce TTATTTCAAAGTCAATACGTTTAGTTTTCTAG(+ACTCAC)GATCCTCTTCCTACACTTAGTGTTTCAGTTT NGG 10 8 17 8 24 0 100 5 10