Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605015109_46ih TTCCGACTTTATCATTTGAGGAGAGAACAAGG(+ACTCAC)GTACCGTCTTGTAATTTTGCCTTTCTTGGTA NGG 10 8 17 8 24 0 100 5 10
    240605015107_o94b AATGTTACCAAGAAAGGCAAAATTACAAGACG(+ACTCAC)GTACCCTTGTTCTCTCCTCAAATGATAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605015106_fy80 ACATTCAGGTCTGAATTCATATCTCAAGAAAG(+ACTCAC)GATCCTCCCTGTCTCTTTTAGTGTCTCATCA NGG 10 8 17 8 24 0 100 5 10
    240605015104_yftj AGCTCTGATGAGACACTAAAAGAGACAGGGAG(+ACTCAC)GATCCTTTCTTGAGATATGAATTCAGACCTG NGG 10 8 17 8 24 0 100 5 10
    240605015103_mzt4 CAGCTAGAGGAATTGTTCCAAGGTTAATAGTG(+ACTCAC)GATCCCCAGATTAGATGTTAAAGAATAGATT NGG 10 8 17 8 24 0 100 5 10
    240605015101_w3rt CATCTAATCTATTCTTTAACATCTAATCTGGG(+ACTCAC)GATCCACTATTAACCTTGGAACAATTCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605015100_gatp CTTAGTCTGGGGTAAAGAGGCTGGTGAAGTAG(+ACTCAC)GTACCTCCAATTGCAGTGTGGTCACTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240605015058_9wo7 ATGGTATTAAAGTGACCACACTGCAATTGGAG(+ACTCAC)GTACCTACTTCACCAGCCTCTTTACCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605015057_z5pe TGAAATTGTCAATTTCAGAGCATGAAGTGAAG(+ACTCAC)GTACCGACTCAAGAAAAAGATTTTTACTGCA NGG 10 8 17 8 24 0 100 5 10
    240605015056_5vak ACTGATGCAGTAAAAATCTTTTTCTTGAGTCG(+ACTCAC)GTACCTTCACTTCATGCTCTGAAATTGACAA NGG 10 8 17 8 24 0 100 5 10